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DOI: 10.1055/s-0028-1099868
A CHEMICAL STUDY OF THE LEAVES OF D. MERTONENSIS, D. VIRIDIFLORA, D. PURPUREA VAR. MINIANA AND D. PARVIFLORA GROWN IN EGYPT
Publication History
Publication Date:
15 January 2009 (online)
Summary
A chemical study of the leaves of Digitalis mertonensis Buxt. & Darl., D. viridiflora Lindl.; D. purpurea L. var. miniana Sampaio and D. parviflora Jacq. has been undertaken.
The preliminary phytochemical screening has shown that the leaves of the four species under investigation yield a crystalline sublimate on microsublimation and contain carbohydrates, cardiac glycosides, tannins of the catechol nature, flavonoids, saponins and oxidase enzyme.
The qualitative investigation using both paper (system: ethyl acetate : benzene : water 84:16:50 + 1 % ethanol to the organic phase) and thin–layer (systems : chloroform : methanol : formamide 80:19 :1, and benzene : ethanol 95 % 7:3) chromatographic techniques has shown that the leaves of each of the four species of Digitalis contain the glycosides: Digitalinum verum, glucolanadoxin, lanatoside B, glucoevatromonoside, lanatoside A, strospeside, gitoxin, digitoxin and acetyl digitoxin. Furthermore the leaves of Digitalis purpurea var. miniana and D. parviflora contain purpurea glycoside B, a glycoside which is present in traces in D. viridiflora but is absent in D. mertonensis. Besides, both D. viridiflora and D. parviflora contain lanatoside C, a glycoside which is absent in the other two species. The glycoside desacetyllanatoside C was detected in the leaves of D. viridiflora only. In addition, varying numbers of unidentified spots appeared in the chromatograms of each of the four species.
The quantitative estimation of the total glycosidal content of each species was determined colorimetrically using Baljet reagent, and calculated in terms of equivalent Digitoxin. The percentage amounts were 1.90 in D. mertonensis, 1.46 in D. viridiflora, 1.40 in D. purpurea var. miniana and 0.775 in D. parviflora.
Zusammenfassung
Die Blätter von Digitalis mertonensis, D. viridiflora, D. purpurea var. miniana und D. parviflora wurden chemisch untersucht.
Durch PC– und DC–Analy,5e wurde in allen vier Arten das Vorkommen von Digitalin/Glucolanadoxin, Lanatosid B, Glucoevatromonosid, Lanatosid A, Stro–spesid, Gitoxin, Digitoxin und Acetyl–Digitoxin sichergestellt.
Purpureaglykosid B war vorhanden in D. purpurea var. miniana und D. parviflora, in Spuren auch in D. viridiflora.
Lanatosid C wurde bei U. viridiflora und D. parviflora gefunden. Desacetyl–lanatosid C kam nur bei D. viridiflora vor.