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Horm Metab Res 2023; 55(02): 136-141
DOI: 10.1055/a-1961-6281
Original Article: Endocrine Research

Genetic and Functional Analyses of the Novel KLF11 Pro193Thr Variant in a Three-Generation Family with MODY7

Gaopeng Guan
1   Department of Endocrinology, Central South University Third Xiangya Hospital, Changsha, China
,
Tiantian Qin
1   Department of Endocrinology, Central South University Third Xiangya Hospital, Changsha, China
,
Li-ling Zhao
1   Department of Endocrinology, Central South University Third Xiangya Hospital, Changsha, China
,
Ping Jin
1   Department of Endocrinology, Central South University Third Xiangya Hospital, Changsha, China
› Author Affiliations Funding Information Research project of Hunan Health Committee — 202103061081 National Natural Science Foundation of China — 81670730 Natural Science Foundation of China Hunan Province — 2021JJ31007 Sinocare Diabetes Foundation — 2020SD09
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Abstract

KLF11 regulates insulin gene expression through binding to the insulin promoter and has been reported as a causative gene for maturity-onset diabetes of the young 7 (MODY7). Here, we report a novel KLF11 variant associated with a three-generation family with early childhood-onset diabetes and explore its clinical and functional characteristics. The three-generational pedigree contains five patients affected by diabetes. The pathogenic variant identified by whole-exome sequencing was further confirmed by Sanger sequencing and pedigree verification. Luciferase reporter assays and glucose-stimulated insulin secretion were used to examine whether the KLF11 variant binds to the insulin promoter and regulate insulin secretion in vitro. The proband, his son, and his uncle exhibited hyperglycemia at ages 32, 13 and 71 years, respectively. All three patients showed characteristics of metabolic syndrome (obesity, dyslipidemia, and diabetes), but the insulin secretion of islet β-cells was impaired. A novel heterozygous missense variant, c.577 C>A (p.Pro193Thr) of the KLF11 gene was detected in all three patients. This variant co-segregates with the diabetes phenotype, consistent with an autosomal dominant disorder. The identified KLF11 p.Pro193Thr variant drastically decreased the transcriptional activity of KLF11, as demonstrated by luciferase reporter assay. Functional analyses revealed that the KLF11 Pro193Thr variant inhibited glucose-stimulated insulin secretion. We identified a novel KLF11 Pro193Thr variant in a three generation family with MODY7. These findings shed light on the molecular mechanisms underlying the pathogenesis of MODY7 and expand the genotype and clinical spectrum of MODY7.

Key words

maturity-onset diabetes of the young 7 - KLF11 - variant

Supplementary Material



Publication History

Received: 01 July 2022

Accepted after revision: 14 October 2022

Accepted Manuscript online:
14 October 2022

Article published online:
24 November 2022

© 2022. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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