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Horm Metab Res 2007; 39(5): 359-365
DOI: 10.1055/s-2007-976540
Original Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Characterization and Transcriptional Regulation of the Human Somatostatin Receptor Subtype 1 Gene

A. Redmann 1 , A. Rasch 2 , H. Tourné 1 , K. Mann 1 , S. Petersenn 1
  • 1Division of Endocrinology, Medical Center, University of Essen, Essen, Germany
  • 2IHF, Institute for Hormone and Fertility Research, University of Hamburg, Hamburg, Germany
Further Information

Publication History

received 17. 7. 2006

accepted 22. 12. 2006

Publication Date:
29 May 2007 (online)

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Abstract

Somatostatin (SRIF) exerts inhibitory effects on virtually all endocrine and exocrine secretions. At least five distinct human SRIF receptors have been identified (sst1-sst5). Analysis of the promoter region may provide tools to understand transcriptional regulation of ssts in various tissues, indicating specific functions. Transcriptional regulation of the human sst1 was analyzed in the present study. Total RNA from a human somatotropic pituitary tumor was reverse transcribed. 5′cDNA regions of the human sst1 were cloned using an adapted inverse PCR method. Among the 15 PCR clones analyzed, 9 demonstrated an extended 5′-utr of 266 nucleotides, determining a thymidine residue as a major transcription start site. No introns were evident in the 5′-utr region. The promoter region lacked consensus sites for TATA or CAAT boxes, YY1, or an initiator sequence, but contained two CpG islands. Different lengths of 5′-flanking regions cloned by PCR were placed upstream of the luciferase reporter gene and transiently transfected into various cell lines. The 2834 nt of the promoter region directed significant transcriptional activity in a somatotropic pituitary cell line, but neither in COS-7 monkey kidney cells nor in AtT-20 murine corticotrope cells. Transcriptional activity was not affected by incubation with various hormones. Several putative transcription factor binding sites inducing the cell-type specific activity were identified.

Key words

somatostatin receptor - G-protein coupled receptor - structure - regulation

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Correspondence

PD Dr. med. S. Petersenn

Division of Endocrinology, Medical Center, University of Essen

Hufelandstraße 55

45122 Essen

Phone: +49/201/723 28 54

Fax: +49/201/723 59 76

Email: stephan.petersenn@uni-due.de

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