Planta Med 2011; 77(5): 461-466
DOI: 10.1055/s-0030-1250407
Natural Product Chemistry
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Protoflavonoids from Ferns Impair Centrosomal Integrity of Tumor Cells

Isabelle Pouny1 , Chantal Etiévant2 , Laurence Marcourt1 , Isabelle Huc-Dumas1 , Muriel Batut1 , Frédéric Girard2 , Michel Wright2 , Georges Massiot1
  • 1Unité Mixte de Service 2597, Centre National de la Recherche Scientifique (CNRS) – Pierre Fabre,, Institut des Sciences et Technologies du Médicament de Toulouse (ISTMT), Toulouse, France
  • 2Unité Mixte de Recherche 2587, Centre National de la Recherche Scientifique (CNRS) – Pierre Fabre, Institut des Sciences et Technologies du Médicament de Toulouse (ISTMT), Toulouse, France
Weitere Informationen

Publikationsverlauf

received June 17, 2010 revised Sept. 10, 2010

accepted Sept. 15, 2010

Publikationsdatum:
13. Oktober 2010 (online)

Abstract

Six protoflavonoids, including two new compounds, were isolated during a large scale screening of fern extracts for original interaction with mitosis. The new compounds isolated from Phegopteris decursive-pinnata and Equisetum fluviatile were 2′,3′-dihydroprotogenkwanone (1) and 2′,3′-dihydro-2′-hydroxyprotoapigenone (2). Known compounds were: protoapigenone, protogenkwanone, protoapigenin, and 4′-O-β-D-glucopyranosyl protoapigenin. They showed a cytotoxic activity against HeLa cells at a micromolar level. IC50 values were 2 µM for compound 1, > 10 µM for compound 2, and respectively 2.4, 0.6, > 10 µM for the known compounds. Their cytotoxic effects were associated with phenotypic changes never observed before and characterized by the loss of centrosomal γ-tubulin labelling in both mitotic and interphasic cells.

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Dr. Georges Massiot

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