TY - JOUR AU - Valle, Ana Catarina Viana; Brunel, Hilana dos Santos Sena; Dallago, Bruno Stéfano Lima; Rodrigues, Lucas Santana; Malard, Patrícia Furtado; da Costa, Rosiane Andrade; Rossetto, Rafael; de Andrade, Rosângela Vieira TI - In-Vitro Growth Kinetics of Mesenchymal Stem Cells in Cytotoxicity Tests Using Low-Diluted Viscum Album SN - 1475-4916 SN - 1476-4245 PY - 2022 JO - Homeopathy JF - Homeopathy LA - EN VL - 112 IS - 01 SP - 040 EP - 049 DA - 2022/08/21 KW - apoptosis KW - cell viability KW - cell differentiation KW - complementary therapy AB - Introduction The use of mesenchymal stem cells (MSC) in cytotoxicity tests is an in-vitro alternative model for predicting initial doses. Homeopathic medicines may stimulate the immune system to combat a pathology effectively and have been used for over two centuries. Viscum album (VA) extracts are widely used in the treatment of cancer, due to their immunomodulatory, cytotoxic and pro-apoptotic properties.Objective This study aimed to evaluate the in-vitro growth kinetics of canine MSC in relation to cytotoxicity, cell differentiation and expression of pluripotentiality markers, using a VA preparation at the D1D2 (1×10−1, 1×10−2 potency (VAD1D2).Methods MSC were obtained from adipose tissue sampled from a healthy dog that was undergoing an elective veterinary procedure and with its owner's permission. The experiments were performed in three groups: MSC treated with VAD1D2 or diluent or untreated (control). The cytotoxicity was evaluated by MTT assay. The differentiation was induced in three lineages, and apoptotic cell labeling was performed by an Annexin-V test.Results At the concentration of 10 μL/mL of VA, the number of cells after in-vitro culture was maintained when compared with the control (untreated) group. A significant and gradual decrease in cell viability was recorded as VA concentrations increased. The apoptosis analysis showed that VA at 20 μL/mL presented absolute percentages of initial apoptosis twice as high as at 10 μL/mL, which was similar to the control (untreated group).Conclusion The results suggest that the use of efficient methods to assess the in-vitro cytotoxicity of VA-based homeopathic medicines using MSC lineages may predict the potential action at different concentrations. These findings demonstrated that VAD1D2 interferes with canine MSC growth kinetics. PB - Georg Thieme Verlag KG DO - 10.1055/s-0042-1747682 UR - http://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0042-1747682 ER -