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Thromb Haemost 2016; 115(06): 1147-1156
DOI: 10.1160/TH15-09-0739
Cellular Haemostasis and Platelets
Schattauer GmbH

Platelet functional and transcriptional changes induced by intralipid infusion

Lea M. Beaulieu   *
1   Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
,
Olga Vitseva   *
1   Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
,
Kahraman Tanriverdi
1   Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
,
Alper Kucukural
2   Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
,
Eric Mick
3   Department of Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, Massachusetts, USA
,
Naomi Hamburg
4   Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
,
Joseph Vita   †
4   Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
,
Jane E. Freedman
1   Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA
› Author Affiliations
Further Information

Publication History

Received: 18 September 2015

Accepted after major revision: 11 February 2016

Publication Date:
27 November 2017 (online)

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Summary

Multiple studies have shown the effects of long-term exposure to high-fat or western diets on the vascular system. There is limited knowledge on the acute effects of high circulating fat levels, specifically on platelets, which have a role in many processes, including thrombosis and inflammation. This study investigated the effects of acute, high-fat exposure on platelet function and transcript profile. Twenty healthy participants were given an intravenous infusion of 20% Intralipid emulsion and heparin over 6 hours. Blood samples were taken prior to and the day after infusion to measure platelet function and transcript expression levels. Platelet aggregation was not significantly affected by Intralipid infusion, but, when mitochondria function was inhibited by carbonyl cyanide 3-chlorophenylhydrazone (CCCP) or oligomycin, platelet aggregation was higher in the post-infusion state compared to baseline. Through RNA sequencing, and verified by RT-qPCR, 902 miRNAs and 617 mRNAs were affected by Intralipid infusion. MicroRNAs increased include miR-4259 and miR-346, while miR-517b and miR-517c are both decreased. Pathway analysis identified two clusters significantly enriched, including cell motility. In conclusion, acute exposure to high fat affects mitochondrial-dependent platelet function, as well as the transcript profile.

Keywords

Clinical studies - gene expression - platelet physiology - obesity

* Co-First Authors.


† Deceased.


 

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