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Thromb Haemost 2014; 112(04): 825-830
DOI: 10.1160/TH14-02-0108
New Technologies, Diagnostic Tools and Drugs
Schattauer GmbH

Performance of two new automated assays for measuring von Willebrand activity: HemosIL AcuStar and Innovance

Emmanuel de Maistre
1   Haematology Laboratory, University Hospital, Dijon, France
,
Fabienne Volot
2   Haemophilia Center, University Hospital, Dijon, France
,
Guillaume Mourey
3   Etablissement Français du Sang, Besançon, France
,
Ludwig S. Aho
4   Epidemiology Department, University Hospital, Dijon, France
,
Catherine Ternisien
5   Haematology Laboratory, University Hospital, Nantes, France
,
Marie-Elisabeth Briquel
6   Haemophilia Center, University Hospital, Nancy, France
,
Marie-Anne Bertrand
7   Haemophilia Center, University Hospital, Besançon, France
,
Brigitte Tardy
8   Haematology Laboratory, University Hospital, Saint Etienne, France
,
Birgit Frotscher
6   Haemophilia Center, University Hospital, Nancy, France
,
Philippe Nguyen
9   Haematology Laboratory, University Hospital, Reims, France
,
Laure Dumont
10   NVH Medicinal, Dijon, France
,
David Vandroux
10   NVH Medicinal, Dijon, France
,
Nathalie Hezard
9   Haematology Laboratory, University Hospital, Reims, France
,
Marc Trossaërt
5   Haematology Laboratory, University Hospital, Nantes, France
› Author Affiliations
Further Information

Publication History

Received: 06 February 2014

Accepted: 30 April 2014

Publication Date:
04 December 2017 (online)

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Summary

The ristocetin cofactor activity assay (VWF:RCo) is the reference method for assessing von Willebrand factor (VWF) activity but remains difficult to perform, and the coefficient of variation of the method is high (about 20–30%). This study evaluated and compared the performance for measuring the VWF activity of two newly commercialised assays [VWF:Ac Innovance (VWF:Ac) and VWF:RCo Acustar (VWF:RCo Acu)] with the reference VWF:RCo aggregation in 123 pathological plasma samples. The correlation and concordance between both new tests (VWF:RCo-Acu and VWF:Ac) and the reference VWF:RCo were good. The results of the VWF activity to VWF antigen ratio were also comparable whatever the method for the classification of VWF deficiency in all patients. Our results showed that both new tests could replace the “gold standard” VWF:RCo in aggregometry with several benefits: they are fully automated, easier and faster to perform, better adapted to emergency situations if necessary.

Keywords

Willebrand activity assay - ristocetin cofactor activity - aggregometry - von Willebrand disease
 

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