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Thromb Haemost 2005; 94(04): 728-737
DOI: 10.1160/TH05-04-0268
Theme Issue Article
Schattauer GmbH

Molecular and phenotypic analyses of human embryonic stem cell-derived cardiomyocytes

Opportunities and challenges for clinical translation
Gareth Goh
1   Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, UK
2   Division of Therapeutics and Molecular Medicine, University of Nottingham, Queens Medical Centre, Nottingham, UK
,
Tim Self
3   Institute of Cell Signalling, University of Nottingham, Queens Medical Centre, Nottingham, UK
,
Maria D. Barbadillo Muñoz
1   Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, UK
4   Division of Obstetrics and Gynaecology, University of Nottingham, Queens Medical Centre, Nottingham, UK
,
Ian P. Hall
2   Division of Therapeutics and Molecular Medicine, University of Nottingham, Queens Medical Centre, Nottingham, UK
,
Lorraine Young
1   Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, UK
4   Division of Obstetrics and Gynaecology, University of Nottingham, Queens Medical Centre, Nottingham, UK
,
Chris Denning
1   Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, UK
› Author AffiliationsFinancial support: This work was funded by MRC and the University of Nottingham.
Further Information

Publication History

Received19 April 2005

Accepted after revision09 July 2005

Publication Date:
07 December 2017 (online)

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Summary

Differentiation of human embryonic stem cells (hESCs) into cardiomyocytes in culture may offer unique opportunities for modeling genetic disorders, screening potentially cardiotoxic pharmaceutical agents or replacing cells of the diseased heart. However, before clinical utility can be realized, numerous hurdles must be overcome. Comprehensive molecular and phenotypic characterization is required but has so far been restricted to cardiomyocytes derived from a limited subset of hESC lines. Thus, we have initiated analysis of cardiomyocyte differentiation and function from a further two independently derived lines, BG01 and HUES-7. The challenge of improving cardiac cell induction, enrichment and maturation must also be addressed to meet the demands of high throughput pharmaceutical screening or to provide sufficient cells to repair an infarcted heart. Transplanted cells must functionally integrate without inducing arrhythmias, while survival and evasion of immune surveillance must be accomplished without tumorigenicity. This review evaluates the opportunities presented by hESC-derived cardiomyocytes and the progress towards surmounting the challenges of clinical translation.

Keywords

Human embryonic stem cells (hESC) - characterization - cardiomyocytes - differentiation - clinical translation
 

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