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Thromb Haemost 2003; 90(05): 796-802
DOI: 10.1160/TH02-09-0013
Blood Coagulation, Fibrinolysis and Cellular Haemostasis
Schattauer GmbH

Intracoronary β2 receptor activation induces dynamic local t-PA release in the pig

Barbro Österlund
1   Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden
,
Christina Jern
2   Clinical Experimental Research Laboratory
3   Institute of Clinical Neuroscience and
,
Helene Seeman-Lodding
4   Anaesthesiology and Intensive Care, Sahlgrenska University Hospital, Göteborg, Sweden
,
Göran Johansson
1   Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden
,
Sören Häggmark
1   Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden
,
Michael Broomé
1   Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden
,
Björn Biber
1   Surgical and Perioperative Science, Anaesthesiology and Intensive care, Umeå University, Umeå, Sweden
› Author AffiliationsGrant support: This study was supported by grants from the Swedish Medical Research Council (project 6575 and 9046), the Swedish Heart-Lung Foundation, the Norrland Heart Fund and the Umeå University, Umeå, Sweden.
Further Information

Publication History

Received 04 September 2002

Accepted after revision 03 July 2003

Publication Date:
05 December 2017 (online)

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Summary

To investigate β2-adrenergic agonist-mediated effects on coronary fluxes of local fibrinolytic factors, healthy anaesthetised and instrumented pigs (n=10) were studied during infusion of isoprenaline (IPR) into the left main coronary artery. Coronary net fluxes of total t-PA antigen, active t-PA and total PAI-1 antigen were determined at baseline and at 3, 5, 7 and 10 minutes of IPR infusion. During IPR, net release of total t-PA increased in a biphasic pattern with transiently high levels at 3 (+440 %) and 7 minutes (+620%) and returned towards baseline at 10 minutes. Net coronary release of active t-PA increased with maximum levels at 3 minutes (+50%). Baseline coronary net flux of total PAI –1 showed a decrease which was most pronounced at 10 minutes. To conclude, a fast β2agonist-mediated local release of t-PA into the coronary vasculature was demonstrated. For total t-PA, this response was characterised by a biphasic release profile.

Keywords

Tissue-type plasminogen activator - plasminogen activator inhibitor - porcine - coronary circulation - β2-adrenergic agonism
 

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