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Planta Med 1982; 44(2): 111-115
DOI: 10.1055/s-2007-971414
Research Articles

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The Effect of Medium Constituents on Growth and Diosgenin Production by Dioscorea deltoidea Cells Grown in Batch Cultures

B. Tal1 , J. Gressel2 , I. Goldberg1
  • 1Department of Applied Microbiology, The Hebrew University, Jerusalem, Israel
  • 2Department of Plant Genetics, The Weizmann Institute, Rehovot, Israel
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Publication History

Publication Date:
29 March 2007 (online)

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Abstract

The effect of nitrogen and carbon sources on growth and diosgenin production was studied with Dioscorea deltoidea cells which were grown under defined conditions in a chemostat, and transferred to batch cultures. Both ammonium and nitrate nitrogen were required for growth and steroid production; diosgenin production was maximal at higher amounts of nitrogen. The nature and concentration of the carbon source (sugar) had marked effects on diosgenin production. A maximal amount of diosgenin (3.8% of dry wt.) was produced on sucrose (15 g/liter) after 21 days of growth in a batch culture.

Key Word Index

Dioscorea deltoidea - Suspension culture - Diosgenin - Nitrogen and carbon effects

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