Production of Forskolin in in vitro Cultures of Coleus forskohlii

Abstract

Shoot tip culture, root organ suspension, and callus culture producing roots were established from shoot tip, root tip, and hypocotyl segments, respectively, on Murashige and Skoog's and White's basal media with or without growth regulators. Forskolin was identified by TLC and HPLC in 60 days old shoot differentiating culture, 30 days old micropropagated plants, and root organ suspension but not in rhizogenic callus. Shoot differentiating culture established in the presence or absence of BAP revealed more or less similar amounts of forskolin as that of the micropropagated plants. Root organ suspension showed traces of forskolin production.

Key words

Coleus forskohlii - forskolin production - shoot tip culture - root organ suspension - callus culture