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Planta Med 1998; 64(8): 775-776
DOI: 10.1055/s-2006-957584
DOI: 10.1055/s-2006-957584
Letters
© Georg Thieme Verlag Stuttgart · New York
In vitro Propagation of Stevia rebaudina Plants Using Multiple Shoot Culture
Further Information
Publication History
1998
1998
Publication Date:
04 January 2007 (online)
Abstract
A multiple shoot culture was induced from nodal segments on MS medium containing half concentration of macroelements, 1% sucrose, and supplemented with NAA (0.01 mg/l). A bioreactor with hormone-free MS medium (300 ml) was inoculated with 1.5 g of the multiple shoot culture and cultivated for a month. The cultivating process of the multiple shoot culture in the bioreactor and the transfer into ex vitro conditions took about 8-9 weeks and produced approx. 600 new seedlings, that could be transferred from greenhouse to field conditions.