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Plant Biol (Stuttg) 2007; 9(1): 77-84
DOI: 10.1055/s-2006-924488
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Subcellular Effects of Drought Stress in Rosmarinus officinalis

E. Olmos1 , M. J. Sánchez-Blanco2 , T. Ferrández2 , J. J. Alarcón2
  • 1Departamento de Nutrición, CEBAS-CSIC, P.O. Box 164, 30100 Espinardo-Murcia, Spain
  • 2Departamento de Riegos, CEBAS-CSIC, P.O. Box 164, 30100 Espinardo-Murcia, Spain
Further Information

Publication History

Received: March 16, 2006

Accepted: July 11, 2006

Publication Date:
28 September 2006 (online)

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Abstract

The use of Rosmarinus officinalis, and other wild plant species, in the Mediterranean area is an interesting solution in order to avoid the desertification and rapid soil erosion, because of their good resistance to environmental conditions. Previous articles have described experiments designed to determine the impact of water stress at the plant level in this species, but more knowledge is required at the subcellular and ultrastructural levels. An anatomic and ultrastructural study of the leaves was conducted on Rosmarinus officinalis plants growing under different water treatments. In the leaves of water-stressed plants, the leaf water potential and turgor decreased, and leaf osmotic potential became more negative with respect to control plants. The anatomic investigations showed that both the mesophyll intercellular spaces and the epidermal cell size were reduced significantly under the more intense drought stress conditions. At the subcellular level, chloroplasts accumulated plastoglobuli and lipid bodies, and cuticle thickness was increased under water stress. In our experiment, the anatomic and ultrastructural modifications of Rosmarinus officinalis could be considered an additional adaptation to drought stress together with physiological and biochemical modifications as antioxidant accumulation.

Key words

Rosmarinus officinalis - chloroplasts - leaf anatomy - water stress - water relations - ultrastructure - plastoglobuli.

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E. Olmos

Departamento de Nutrición
CEBAS-CSIC

P.O. Box 164

30100 Espinardo-Murcia

Spain

Email: eolmos@cebas.csic.es

Editor: M. Riederer

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