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DOI: 10.1055/s-2005-872991
Georg Thieme Verlag KG Stuttgart · New York
Möglichkeiten und Grenzen der Mikrogefäßdarstellung
Microvessel Visualisation: Applications and LimitationsPublication History
Eingang des Manuskriptes: 19.10.2005
Angenommen: 24.10.2005
Publication Date:
02 January 2006 (online)
Zusammenfassung
Für Untersuchungen des Gefäßsystems und der terminalen Strombahn steht eine Reihe komplementärer morphologischer Untersuchungsverfahren zur Verfügung, die zu einer verbesserten Struktur-Funktions-Analyse eingesetzt werden. Zweidimensionale licht- und elektronenoptische Schnittverfahren mit oder ohne spezifische Markierungen werden durch dreidimensionale Visualisierungsmöglichkeiten der Vaskularisation und der Perfusion ergänzt. Noch dominieren die statischen, Ex-vivo-Untersuchungsmethoden, die Zukunft wird jedoch von intravitalen Methoden bestimmt werden.
Abstract
Vessel systems and the microvascular unit may be studied by a variety of morphological techniques which enable improved structure-function analyses. Two-dimensional light and electron microscopic sectioning techniques with and without specific markers are complemented by 3D vascularisation and perfusion study techniques. Static ex vivo modalities still prevail, however, the future will be dominated by intravital high-resolution techniques.
Schlüsselwörter
Angiogenese - Morphologie - Immunohistochemie - Gefäßdichte - konfokale Mikroskopie - Corrosion Casting - Elektronenmikroskopie
Key words
Angiogenesis - morphology - immunostaining - microvessel density count - confocal microscopy - corrosion casting - electron microscopy
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Univ.-Prof. Dr. med. M. A. Konerding
Institut für Anatomie und Zellbiologie
Johannes-Gutenberg-Universität Mainz
Saarstraße 13 - 18
55099 Mainz
Email: konerdin@uni-mainz.de