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Exp Clin Endocrinol Diabetes 2005; 113(2): 102-106
DOI: 10.1055/s-2004-830538
Article

J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Decreased Expression of G-Protein Coupled Receptor Kinase 2 in Cold Thyroid Nodules

C. Voigt1 , H.-P. Holzapfel1 , R. Paschke1
  • 1III. Medical Department, University of Leipzig, Leipzig, Germany
Further Information

Publication History

Received: December 2, 2003 First decision: February 16, 2004

Accepted: June 28, 2004

Publication Date:
17 March 2005 (online)

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Abstract

G-protein coupled receptor kinases (GRKs) have been shown to regulate the homologous desensitization of different G-protein coupled receptors. We have previously demonstrated that the expression of GRK 3 and 4 is increased in hyperfunctioning thyroid nodules (HTNs) and that GRKs 2, 3, 5 and 6 are able to desensitize the TSHR in vitro. Since cold thyroid nodules (CTNs) and HTNs show different molecular and functional properties, different expression patterns of GRKs in these nodules can be expected. The comparison of GRK expression between CTNs and HTNs could give additional insight into the regulation mechanisms of these nodules. We therefore examined the expression of GRKs in CTNs and analyzed the differences to HTNs. The expression of the different GRKs in CTNs was measured by Western blot followed by chemiluminescence imaging.

We found a decreased expression of GRK 2 in CTNs compared to their surrounding tissues and an increased expression of GRK 3 and 4 in CTNs, which is similar to HTNs. The decreased GRK 2 expression most likely results from reduced cAMP stimulation in CTNs. However, the increased GRK 3 and 4 expression in CTNs remains unclear and requires further investigations.

Key words

GRK - TSH receptor - thyroid - nodule - protein expression

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M.D. R. Paschke

III. Medical Department, University of Leipzig

Ph. Rosenthal Str. 27

04103 Leipzig

Germany

Phone: + 493419713200

Fax: + 49 34 19 71 32 09

Email: pasr@medizin.uni-leipzig.de

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