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Plant Biol (Stuttg) 2004; 6(4): 440-446
DOI: 10.1055/s-2004-817958
Original Paper

Georg Thieme Verlag Stuttgart KG · New York

QTL Mapping for a Trade-Off between Leaf and Bud Production in a Recombinant Inbred Population of Microseris douglasii and M. bigelovii (Asteraceae, Lactuceae): A Potential Preadaptation for the Colonization of Serpentine Soils

O. Gailing1 , M. R. Macnair2 , K. Bachmann3
  • 1Institute of Forest Genetics and Forest Tree Breeding, Georg August University Göttingen, Göttingen, Germany
  • 2Hatherly Laboratories, Department of Biological Sciences, University of Exeter, Prince of Wales Road, Exeter, EX4 6EZ, UK
  • 3Institute of Plant Genetics and Crop Plant Research (IPK-Gatersleben), Corrensstraße 3, 06466 Gatersleben, Germany
Further Information

Publication History

Publication Date:
12 July 2004 (online)

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Abstract

The different response to growth on serpentine soil is a major autecological difference between the annual asteracean species Microseris douglasii and M. bigelovii, with nearly non-overlapping distribution ranges in California. Early flowering and seed set is regarded as a crucial character contributing to escape drought and thus is strongly correlated with survival and reproductive success on serpentine as naturally toxic soil. M. bigelovii (strain C94) from non-serpentine soil produces more leaves at the expense of bud production in the first growing phase than M. douglasii (B14) from serpentine soil. A QTL mapping study for this trade-off and for other growth-related traits was performed after six generations of inbreeding (F7) from a single interspecific hybrid between B14 and C94 on plants that were grown on serpentine and alternatively on normal potting soil. The trade-off is mainly correlated with markers on one map region on linkage group 03a (lg03a) with major phenotypic effects (phenotypic variance explained [PVE] = 18.8 - 31.7 %). Plants with the M. douglasii allele in QTL-B1 (QTL-NL1) produce more buds but fewer leaves in the first 119 days on both soil types. Three modifier QTL could be mapped for bud and leaf production. In one modifier (QTL-B2 = QTL-NL4) the M. douglasii allele is again associated with more buds but fewer leaves. QTL mapped for bud set in the F6 co-localize with QTL-B1 (major QTL) and QTL-B3. Two additional QTL for leaf length and red coloration of leaves could be mapped to one map region on lg03a. Co-localization of the two QTL loci with major phenotypic effects on bud and leaf production strongly suggests that a major genetic locus controls the trade-off between the two adaptive traits. The importance of mutational changes in major genes for the adaptation to stressful environments is discussed.

Key words

Trade-off - serpentine soil - QTL - ecological genetics - Microseris.

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O. Gailing

Institute of Forest Genetics and Forest Tree Breeding
Georg August University Göttingen

Büsgenweg 2

37077 Göttingen

Germany

Email: ogailin@gwdg.de

Section Editor: F. Salamini

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