The Bioconversion Process of Deoxypodophyllotoxin with Linum flavum Cell Cultures

Abstract

The in vitro cell suspension culture of Linum flavum is able to convert high amounts of the 2,7′-cyclolignan deoxypodophyllotoxin into 6-methoxypodophyllotoxin 7-O-glucoside. We studied this conversion in detail by monitoring the intermediates and side-products after feeding different concentrations of deoxypodophyllotoxin. At a low concentration (0.1 mM) deoxypodophyllotoxin is rapidly converted into 6-methoxypodophyllotoxin 7-O-glucoside, 6-methoxypodophyllotoxin and traces of β-peltatin and podophyllotoxin. The feeding of 0.5 and 2.0 mM also shows a rapid conversion into 6-methoxypodophyllotoxin 7-O-glucoside, but a delayed formation of 6-methoxypodophyllotoxin and β-peltatin. By using different extraction methods we delivered proof in favour of the hypothesis that a part of the deoxypodophyllotoxin after uptake is temporarily stored as β-peltatin glucoside.

Key words

Linum flavum - Linaceae - bioconversion - deoxypodophyllotoxin - lignans - podophyllotoxin

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