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Klin Monbl Augenheilkd 2001; 218(5): 373-375
DOI: 10.1055/s-2001-15904
EXPERIMENTELLE STUDIE

Georg Thieme Verlag Stuttgart · New York

Latanoprost is a vasoconstrictor in isolated porcine ciliary arteries

Vasokonstriktorische Eigenschaften von Latanoprost an isolierten Ziliararterien des SchweinesGiovanni Brogiolo, Josef Flammer, Ivan  O. Haefliger
  • Laboratory of Ocular Pharmacology and Physiology, University Eye Clinic Basel, Mittlere Strasse 91, PO Box,
  • CH-4012 Basel, Switzerland
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Publication History

Publication Date:
31 December 2001 (online)

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Fragestellung Die vorliegende pharmakologische Studie wurde durchgeführt, um die vasokonstriktorische Wirkung von Latanoprost (Prostaglandin F2α-Analogon) an isolierten Ziliararterien des Schweines zu evaluieren.

Material und Methoden An Ziliararterien wurde mittels eines Myographsystems die isometrische Kraft bei kumulativ zunehmenden Konzentrationen (0,1 nM - 0,1 mM) von Latanoprost gemessen. Die Experimente wurden an Arterien im Ruhezustand wie auch an Kaliumchlorid (KCl; 40 mM) oder Endothelin-1 (10 nM) präkontrahierten Ziliararterien durchgeführt. Kontraktionen wurden in Prozent einer durch 100 mM KCl induzierten Kontraktion exprimiert.

Ergebnisse Latanoprost zeigte eine signifikante (p < 0,001), konzentrationsabhängige Zunahme der Kontraktionskraft bei Ziliararterien im Ruhezustand (Maximum: 67,5 ± 3,1 %, n=5; PD50: 5.7 ± 0.1). In KCl oder in Endothelin-1 präkontrahierten Ziliararterien bewirkte Latanoprost keine Relaxation, sondern auch signifikante (p < 0,001) Kontraktionen (Maximum: 32,8 ± 4,0 %, n=5 resp. 25,6 ± 5,5 %, n=8).

Schlussfolgerung Latanoprost zeigt an isolierten Ziliararterien des Schweines vasokonstriktorische und keine vasorelaxierende Eigenschaften, wobei die klinische Relevanz dieser Beobachtung bei der Behandlung von Glaukompatienten noch untersucht werden muss.

Abstract

Purpose The purpose of this study was to assess the vasoconstrictor effect of the prostaglandin F2α analog latanoprost in isolated porcine ciliary arteries.

Material and Methods With a myograph system to measure isometric forces, porcine ciliary arteries were exposed, in a cumulative manner, to increasing concentrations of latanoprost (0.1 nM - 0.1 mM). Experiments were conducted in quiescent vessels as well as in vessels precontracted with potassium chloride (KCl; 40 mM) or endothelin-1 (10 nM). Contractions were expressed in percent of 100 mM KCl-induced contraction.

Results In a concentration dependent manner latanoprost induced significant (p < 0.001) contractions in quiescent vessels (maximum: 68 ± 3 %, n=5; PD50: 5.7 ± 0.1). In KCl or in endothelin-1 precontracted vessels latanoprost did not evoke any relaxation but in contrast significant (p < 0.001) contractions (maximum: 33 ± 4 %, n=5 and 26 ± 6 %, n=8, respectively).

Conclusions Latanoprost has a vasoconstrictor effect in isolated porcine ciliary arteries. No relaxation could be observed. The clinical relevance of this finding requires further evaluation.

Schlüsselwörter

Intraokulardruck - okulärer Blutfluss - vaskuläre Risikofaktoren - Normaldruck-Glaukom - Xalatan

Key words

Intraocular pressure - normal tension glaucoma - ocular blood flow - vascular risk factor - xalatan

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