Download PDF
J Pediatr Intensive Care 2020; 09(04): 265-270
DOI: 10.1055/s-0040-1710587
Original Article

The Ameliorative Effects of Ascorbic Acid on Critical Illness Polyneuropathy in Rodent Sepsis Model

Eda Sunnetci Silistre
1   Department of Pediatric, Istanbul Training and Research Hospital, Istanbul, Turkey
,
Oytun Erbas
2   Department of Physiology, Bilim University Medical Faculty, Istanbul, Turkey
› Author Affiliations Funding None.
› Further Information
    Permissions and Reprints

Abstract

Although the exact pathophysiology of critical illness polyneuropathy (CIP) is still unknown, there are several hypotheses, some of which are increased inflammation and oxidative stress. We used rodent sepsis model in which we induced sepsis through cecal ligation followed by cecal puncture. We then administered ascorbic acid (AA) and evaluated outcomes. The levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α), interleukins (IL)-6 in the plasma, and heat shock protein-70 (HSP-70) levels in the sciatic nerve were measured, and also electromyography analyses were performed. While plasma MDA, TNF-α, and IL-6 levels were decreased significantly with AA treatment, sciatic nerve levels of HSP-70 were significantly elevated in the AA group. A significant increase in compound muscle action potential (CMAP) amplitude and a significant decrease in CMAP latency were detected in the AA group. We observed healing effects of AA on a rat model of CIP and these effects seem to be related to its anti-inflammatory and antioxidant properties.

Keywords

critical illness polyneuropathy - sepsis - ascorbic acid - malondialdehyde - compound muscle action potential


Publication History

Received: 11 March 2020

Accepted: 15 April 2020

Article published online:
22 May 2020

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Stuttgart · New York

 

  • References

  • 1 Latronico N, Bolton CF. Critical illness polyneuropathy and myopathy: a major cause of muscle weakness and paralysis. Lancet Neurol 2011; 10 (10) 931-941
    CrossrefPubMedGoogle Scholar
  • 2 Erbaş O, Yeniel AO, Akdemir A. et al. The beneficial effects of levetiracetam on polyneuropathy in the early stage of sepsis in rats: electrophysiological and biochemical evidence. J Invest Surg 2013; 26 (06) 312-318
    CrossrefPubMedGoogle Scholar
  • 3 Erbaş O, Ergenoglu AM, Akdemir A, Yeniel AO, Taskiran D. Comparison of melatonin and oxytocin in the prevention of critical illness polyneuropathy in rats with experimentally induced sepsis. J Surg Res 2013; 183 (01) 313-320
    CrossrefPubMedGoogle Scholar
  • 4 Solmaz V, Aksoy D, Yılmaz M, Eser E, Erbas O. Demonstration of ameliorative effect of lacosamide: in a rat model of sepsis-induced critical illness polyneuropathy. Neurol Res 2015; 37 (09) 797-802
    CrossrefPubMedGoogle Scholar
  • 5 Sarge KD, Murphy SP, Morimoto RI. Activation of heat shock gene transcription by heat shock factor 1 involves oligomerization, acquisition of DNA-binding activity, and nuclear localization and can occur in the absence of stress. Mol Cell Biol 1993; 13 (03) 1392-1407
    CrossrefPubMedGoogle Scholar
  • 6 Aschkenasy G, Bromberg Z, Raj N, Deutschman CS, Weiss YG. Enhanced Hsp70 expression protects against acute lung injury by modulating apoptotic pathways. PLoS One 2011; 6 (11) e26956
    CrossrefPubMedGoogle Scholar
  • 7 Lyons MM, Raj NN, Chittams JL, Kilpatrick L, Deutschman CS. TAT-HSP70 attenuates experimental lung injury. Shock 2015; 43 (06) 582-588
    CrossrefPubMedGoogle Scholar
  • 8 Albokhadaim IF, Althnaian TA, El-Bahr SM. Gene expression of heat shock proteins/factors (HSP60, HSP70, HSP90, HSF-1, HSF-3) and antioxidant enzyme activities in heat stressed broilers treated with vitamin C. Pol J Vet Sci 2019; 22 (03) 565-572
    PubMedGoogle Scholar
  • 9 Passage E, Norreel JC, Noack-Fraissignes P. et al. Ascorbic acid treatment corrects the phenotype of a mouse model of Charcot-Marie-Tooth disease. Nat Med 2004; 10 (04) 396-401
    CrossrefPubMedGoogle Scholar
  • 10 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254
    CrossrefPubMedGoogle Scholar
  • 11 Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: analytical and biological challenges. Anal Biochem 2017; 524: 13-30
    CrossrefPubMedGoogle Scholar
  • 12 Davey MW, Stals E, Panis B, Keulemans J, Swennen RL. High-throughput determination of malondialdehyde in plant tissues. Anal Biochem 2005; 347 (02) 201-207
    CrossrefPubMedGoogle Scholar
  • 13 Apostolopoulou K, Konstantinou D, Alataki R. et al. Ischemia-reperfusion injury of sciatic nerve in rats: protective role of combination of vitamin C with E and tissue plasminogen activator. Neurochem Res 2018; 43 (03) 650-658
    CrossrefPubMedGoogle Scholar
  • 14 Riffel APK, Santos MCQ, de Souza JA. et al. Treatment with ascorbic acid and α-tocopherol modulates oxidative-stress markers in the spinal cord of rats with neuropathic pain. Braz J Med Biol Res 2018; 51 (04) e7097
    CrossrefPubMedGoogle Scholar
  • 15 Li L, Li Y, Fan Z. et al. Ascorbic acid facilitates neural regeneration after sciatic nerve crush injury. Front Cell Neurosci 2019; 13: 108
    CrossrefPubMedGoogle Scholar
  • 16 Yun SH, Moon YS, Sohn SH, Jang IS. Effects of cyclic heat stress or vitamin C supplementation during cyclic heat stress on HSP70, inflammatory cytokines, and the antioxidant defense system in Sprague Dawley rats. Exp Anim 2012; 61 (05) 543-553
    CrossrefPubMedGoogle Scholar
  • 17 Yin B, Tang S, Sun J. et al. Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress. Cell Stress Chaperones 2018; 23 (04) 735-748
    CrossrefPubMedGoogle Scholar
  • 18 Zhou G, Yan M, Guo G, Tong N. Ameliorative effect of berberine on neonatally induced type 2 diabetic neuropathy via modulation of BDNF, IGF-1, PPAR-γ, and AMPK expressions. Dose Response 2019; 17 (03) 1559325819862449
    CrossrefPubMedGoogle Scholar
  • 19 Son EW, Mo SJ, Rhee DK, Pyo S. Vitamin C blocks TNF-alpha-induced NF-kappaB activation and ICAM-1 expression in human neuroblastoma cells. Arch Pharm Res 2004; 27 (10) 1073-1079
    CrossrefPubMedGoogle Scholar
  • 20 Kawade N, Tokuda Y, Tsujino S. et al. Dietary intake of ascorbic acid attenuates lipopolysaccharide-induced sepsis and septic inflammation in ODS rats. J Nutr Sci Vitaminol (Tokyo) 2018; 64 (06) 404-411
    CrossrefPubMedGoogle Scholar
  • 21 Gess B, Baets J, De Jonghe P, Reilly MM, Pareyson D, Young P. Ascorbic acid for the treatment of Charcot-Marie-Tooth disease. Cochrane Database Syst Rev 2015; (12) CD011952
    PubMedGoogle Scholar