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Thorac Cardiovasc Surg 2019; 67(06): 494-501
DOI: 10.1055/s-0038-1675398
Original Basic Science
Georg Thieme Verlag KG Stuttgart · New York

Influence of Medication-Induced Preconditioning or Remote Ischemic Preconditioning on the Intrinsic Vascular Extracellular RNA/Ribonuclease System in Cardioprotection

Konstantin J. Tolkmitt
1   Department of Cardiovascular Surgery, Medical School, Justus-Liebig-University, Giessen, Germany
,
Sakine Simsekyilmaz
2   Institute of Pharmacology and Clinical Pharmacology, Universitatsklinikum Dusseldorf, Dusseldorf, Germany
,
Julia Schipke
3   Medizinische Hochschule Hannover, Institute of Functional and Applied Anatomy, Hannover, Germany
4   Cluster of Excellence REBIRTH (Regenerative Biology to Reconstructive Therapy), Hannover, Germany
,
Christian Mühlfeld
3   Medizinische Hochschule Hannover, Institute of Functional and Applied Anatomy, Hannover, Germany
4   Cluster of Excellence REBIRTH (Regenerative Biology to Reconstructive Therapy), Hannover, Germany
,
Klaus T. Preissner
5   Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany
,
Andreas Böning
6   Department of Cardiovascular Surgery, University Hospital Giessen, Giessen, Germany
› Author Affiliations
Further Information

Publication History

13 June 2018

21 June 2018

Publication Date:
16 November 2018 (online)

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Abstract

Background It has been demonstrated that remote ischemic preconditioning (RIPC) increases ribonuclease (RNase) levels and protects the heart by reducing extracellular ribonucleic acid (eRNA). As medication-induced preconditioning (MIPC) is also a powerful tool for cardioprotection, we examined the influence of both types of preconditioning on the eRNA/RNase system.

Methods In 17 male rats, RIPC (3 × 5 minute hind-leg ischemia) or MIPC (isoflurane and buprenorphine anesthesia) was performed. Five rats served as control and did not undergo preconditioning (non-MIPC). After preconditioning, eRNA levels and RNase activity were determined in plasma, and the hearts were mounted on a blood-perfused Langendorff ischemia/reperfusion apparatus. Hemodynamic, metabolic, and electron microscopic parameters were determined. Furthermore, MIPC with one anesthetic drug only (isoflurane, buprenorphine, or etomidate) was induced in another five rats. After 30 minutes, eRNA levels and RNase activity were determined and compared with an RIPC group (n = 5).

Results The plasma of RIPC-treated rats had higher RNase activity and lower eRNA levels than that of MIPC-treated rats. In addition, RIPC increased RNase activity more than MIPC with one drug alone. The RNase activity and eRNA levels in these MIPC groups differed considerably. Hemodynamic parameters of RIPC- and MIPC-treated hearts were better preserved after 90-minute ischemia than those of non-MIPC hearts. No obvious differences were noted between MIPC and RIPC regarding hemodynamics, metabolism, or structural parameters.

Conclusions Our results suggest that RIPC does not have any additional cardioprotective benefit in this experimental system. However, the influence of RIPC on the eRNA/RNase system was greater than that of MIPC.

Keywords

myocardial protection/cardioplegia - ischemia/reperfusion - organ preservation - anesthesia

Note

Presented at the 47th annual meeting of the DGTHG (Deutsche Gesellschaft für Thorax-, Herz- und Gefäßchirurgie), Leipzig, February 18, 2018.


Comment to this article:

Regulation of Animal Research in Science: How to Harm Both Science and Animal ProtectionThorac Cardiovasc Surg 2019; 67(06): 502-502
DOI: 10.1055/s-0038-1675379

 

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