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Thromb Haemost 1997; 78(06): 1500-1504
DOI: 10.1055/s-0038-1665441
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Schattauer GmbH Stuttgart

Presence of P2X1 Purinoceptors in Human Platelets and Megakaryoblastic Cell Lines

Catherine Vial
The INSERM U.311, ETSS, Strasbourg, France
,
Béatrice Hechier
The INSERM U.311, ETSS, Strasbourg, France
,
Catherine Léon
The INSERM U.311, ETSS, Strasbourg, France
,
Jean-Pierre Cazenave
The INSERM U.311, ETSS, Strasbourg, France
,
Christian Gachet
The INSERM U.311, ETSS, Strasbourg, France
› Author Affiliations
Further Information

Publication History

Received 31 1996

Accepted after resubmission 08 August 1997

Publication Date:
12 July 2018 (online)

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Summary

Human platelets are thought to possess at least two subtypes of purinoceptor, one of which, coupled to G-proteins, could be the P2Y1 receptor (Léon et al. 1997). However, it has been suggested that the unique rapid calcium influx induced by ADP in platelets could involve P2X1 ionotropic receptors (MacKenzie et al. 1996) and the aim of this study was thus to investigate the presence of P2X purinoceptors in platelets and megakaryoblastic cells. Using PCR experiments, we found P2X1 mRNA to be present in human platelets and megakaryoblastic cell lines. In platelets, the selective P2X1 agonist αβMeATP induced a rise in intracellular calcium only in the presence of external calcium and this effect was antagonized by suramin and PPADS. Repeated addition of a�MeATP desensitized the P2X1 purinoceptor but only slightly affected the ADP response, while no calcium response to αβMeATP was observed in megakaryoblastic cells. These results support the existence of functional P2X1 purinoceptors on human platelets and the presence of P2X1 transcripts in megakaryoblastic cell lines.

 

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