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Thromb Haemost 1997; 78(05): 1417-1419
DOI: 10.1055/s-0038-1665423
Letter to the Editor
Schattauer GmbH Stuttgart

Ratiometric Measurements with Fluorescent Indicators Should Be Interpreted Cautiously in Studies of Platelet Calcium Signaling for von Willebrand Factor

John C Kermode
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi, USA
,
Qi Zheng
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi, USA
,
Elizabeth P Milner
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi, USA
› Author Affiliations
Further Information

Publication History

Received 09 1997

Accepted after resubmission 09 July 1997

Publication Date:
12 July 2018 (online)

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  • References

  • 1 Kroll MH, Harris TS, Moake JL, Handin RI, Schafer AI. von Willebrand factor binding to platelet Gplb initiates signals for platelet activation. J Clin Invest 1991; 88: 1568-1573
    CrossrefPubMedGoogle Scholar
  • 2 Chow TW, Heliums JD, Moake JL, Kroll MH. Shear stress-induced von Willebrand factor binding to platelet glycoprotein lb initiates calcium influx associated with aggregation. Blood 1992; 80: 113-120
    PubMedGoogle Scholar
  • 3 Ikeda Y, Handa M, Kamata T, Kawano K, Kawai Y, Watanabe K, Kawaka-mi K, Sakai K, Fukuyama M, Itagaki I, Yoshioka A, Ruggeri ZM. Transmembrane calcium influx associated with von Willebrand factor binding to GP lb in the initiation of shear-induced platelet aggregation. Thromb Haemost 1993; 69: 496-502
    Article in Thieme ConnectPubMedGoogle Scholar
  • 4 Francesconi MA, Deana R, Girolami A, Pontara E, Casonato A. Platelet aggregation induced by plasma from type HB von Willebrand’s disease patients is associated with an increase in cytosolic Ca2+concentration. Thromb Haemost 1993; 70: 697-701
    Article in Thieme ConnectPubMedGoogle Scholar
  • 5 Ozaki Y, Satoh K, Yatomi Y, Miura S, Fujimura Y, Kume S. Protein tyrosine phosphorylation in human platelets induced by interaction between glycoprotein lb and von Willebrand factor. Biochim Biophys Acta 1995; 1243: 482-488
    CrossrefPubMedGoogle Scholar
  • 6 Mazzucato M, De MarcoL, Pradella P, Masotti A, Pareti FI. Porcine von Willebrand factor binding to human platelet GPIb induces transmembrane calcium influx. Thromb Haemost 1996; 75: 655-660
    Article in Thieme ConnectPubMedGoogle Scholar
  • 7 Kermode JC, Zheng Q, Cook EP. Fluorescent indicators give biased estimates of intracellular free calcium change in aggregating platelets: implication for studies with human von Willebrand factor. Blood Cells Mol Dis 1996; 22: 238-253
    CrossrefPubMedGoogle Scholar
  • 8 Tepel M, Wischniowski H, Zidek W. Thapsigargin-induced [Ca2+]1increase activates sodium influx in human platelets. Biochim Biophys Acta 1994; 1220: 248-252
    CrossrefPubMedGoogle Scholar