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Thromb Haemost 1985; 53(03): 381-385
DOI: 10.1055/s-0038-1661319
Original Article
Schattauer GmbH Stuttgart

A Modification of the Wu and Hoak Method for the Determination of Platelet Aggregates and Platelet Adhesion

Sudhir K Bowry
1   The Bioengineering Unit, University of Strathclyde, Glasgow, Scotland
,
Colin R M Prentice
2   The Department of Medicine, The General Infirmary, Leeds, England
,
J M Courtney
1   The Bioengineering Unit, University of Strathclyde, Glasgow, Scotland
› Author Affiliations
Further Information

Publication History

Received 31 January 1984

Accepted 21 March 1985

Publication Date:
18 July 2018 (online)

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Summary

The Wu and Hoak method for determining circulating platelet aggregates has poor reproducibility; problems have been reported with the composition of the buffer systems, haemolysis, the effects of blood collection technique and a divergence of the platelet aggregate ratio in blood for healthy donors from the theoretical value of 1. Our investigations suggest that the original technique is highly operator-dependent, especially the collection of blood and the method of counting platelets after centrifugation. We describe an improved modification of the Wu and Hoak technique; a new buffer system has been developed and the proportion of blood in the buffered EDTA and buffered EDTA- formalin solutions has been altered to obtain platelet rich plasma. The platelet aggregate ratio (PAR) by this modified method for healthy donors in two different studies was 0.97 ± 0.02 and 0.98 ± 0.01 respectively. Finally, the principle of Wu and Hoak was used to measure accurately platelet adhesion, without the role of platelet-platelet interactions (aggregation). Platelet adhesion and aggregation were then used to evaluate the thrombogenicity of various artificial surfaces, including silicone rubber and polytetra- fluoroethylene (PTFE) vascular grafts.

Keywords

Platelet aggregation - Adhesion - Artificial surfaces
 

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