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Thromb Haemost 1985; 53(02): 188-194
DOI: 10.1055/s-0038-1661270
Original Article
Schattauer GmbH Stuttgart

The Electrophoretic Mobility Heterogeneity of Human Platelet Subpopulations of Different Buoyant Densities

Stephanie M Jung
The Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Tokyo, Japan
,
Kenjiro Tanoue
The Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Tokyo, Japan
,
Hiroh Yamazaki
The Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Honkomagome, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

Received 27 September 1984

Accepted 07 December 1984

Publication Date:
18 July 2018 (online)

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Summary

Human platelets were separated into density subpopulations by using a step-wise gradient of Percoll in Tris-NaCl buffer. The absolute value of the electrophoretic mobility (EPM) of the density subpopulations was found to be a linear function of the density of the platelets, with EPM becoming less negative with increasing platelet density. Platelet volume distributions, mode volume, and sialic acid and protein contents were found to increase with platelet density, while no differences were found in GPU, GPIII, and GPIV contents among the subpopulations. An estimate of charge density was made from the ratio between the PAS-staining material (membrane GP’s) and platelet surface area. The ratio was found to decrease as platelet density increased, consistent with the less negative EPM values observed for the higher density platelets. This lower surface charge of heavier platelets, which would lower charge repulsion between cells, agrees with the premise that heavier platelets are more active.

Keywords

Platelet density - Surface negative charge - Glycoproteins - Sialic acid - Heterogeneity of platelets
 

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