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Thromb Haemost 1984; 52(03): 281-287
DOI: 10.1055/s-0038-1661196
Original Article
Schattauer GmbH Stuttgart

Assessment of Anti-Streptokinase Antibody Levels in Human Sera Using a Microradioimmunoassay Procedure

D M Moran
The Beecham Pharmaceuticals Research Division, Biosciences Research Centre, Epsom, Surrey, U.K.
,
R Standring
The Beecham Pharmaceuticals Research Division, Biosciences Research Centre, Epsom, Surrey, U.K.
,
E A Lavender
The Beecham Pharmaceuticals Research Division, Biosciences Research Centre, Epsom, Surrey, U.K.
,
G S Harris
The Beecham Pharmaceuticals Research Division, Biosciences Research Centre, Epsom, Surrey, U.K.
› Author Affiliations
Further Information

Publication History

Received 18 June 1984

Accepted 31 August 1984

Publication Date:
19 July 2018 (online)

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Summary

A high capacity, quantitative radioimmunoassay procedure has been developed to measure IgG antibodies to streptokinase (SK) in human serum. Results showed that the use of low concentrations of 125I-SK (100 ng/ml) and ambient temperature incubation conditions minimised degradation of the target labelled antigen and afforded antibody binding values which could be confidently related to the native intact SK protein. SK-complexed to plasminogen was also shown to retain the equivalent antigenic activity of native SK. Comparison of the absolute anti-SK levels with streptokinase resistance titres demonstrated that the two measurements of antibody correlated statistically but afforded quantitatively poor agreements. A survey of IgG levels to SK in sera from 93 normal healthy volunteers showed that all individuals displayed readily measurable anti-SK antibodies, with some 80% having IgG concentration capable of binding in excess of 1 μg SK per ml serum.

Key words

Anti-streptokinase antibodies - Streptokinase antigenicity - Streptokinase fragmentation - Streptokinase complexes - plasminogen complexes
 

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