Download PDF
Thromb Haemost 1984; 52(02): 102-104
DOI: 10.1055/s-0038-1661149
Original Article
Schattauer GmbH Stuttgart

The Mesothelial Cell as a Non-Thrombogenic Surface

L J Nicholson
The Departments of Oncology, Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U.K.
,
J M F Clarke
The Departments of Oncology, Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U.K.
,
R M Pittilo
The Departments of Oncology, Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U.K.
,
S J Machin
The Departments of Oncology, Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U.K.
,
N Woolf
The Departments of Oncology, Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U.K.
› Author Affiliations
Further Information

Publication History

Received 20 February 1984

Accepted 12 June 1984

Publication Date:
19 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

A technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

Keywords

Mesothelial cell - Cell culture - Scanning electron microscopy - Platelets - Artificial circulation - Vascular prosthesis
 

  • References

  • 1 Berger K, Sauvage LR, Rao AM, Wood SJ. Healing of arterial prostheses in man; its incompleteness. Ann Surg 1972; 175: 118-127
    CrossrefPubMedGoogle Scholar
  • 2 Herring MB, Gardner A, Glover J. A single staged technique for seeding vascular grafts with autogenous endothelium. Surgery 1978; 84: 498-504
    PubMedGoogle Scholar
  • 3 Graham LM, Vinter DW, Ford JW, Khan RH, Burkel WE, Stanley JC. Endothelial seeding of prosthetic vascular grafts: early experi-mental studies with cultured autologous canine endothelium. Arch Surg 1980; 115: 929-933
    CrossrefPubMedGoogle Scholar
  • 4 Clarke JM F, Pittilo RM, Machin SJ, Woolf N. A study of the possible role of mesothelium as a surface for flowing blood. Thromb Haemostas 1984; 51: 57-60
    PubMedGoogle Scholar
  • 5 Turitto VT, Baumgartner HR. Platelet Adhesion. In: Measurements of Platelet Function. Methods in Haematology. Harker LA, Zimmerman TS. (eds) Churchill Livingstone; Edinburgh - London - Melbourne - New York: 1983. 8 46-63
    Google Scholar
  • 6 Dobbie JW, Zaki M, Wilson L. Ultrastructural studies on the peritoneum with special reference to chronic ambulatory peritoneal dialysis. Scott Med J 1981; 26: 213-223
    CrossrefPubMedGoogle Scholar
  • 7 Watters WB, Buck RC. Scanning Electron microscopy of mesothelial regeneration in the rat. Lab Invest 1972; 26: 604-609
    PubMedGoogle Scholar
  • 8 Madison LD, Bergstrom-Porter B, Torrs R, Shelton E. Regulation of surface topography of mouse peritoneal cells. J Cell Biol 1979; 82: 783-797
    CrossrefPubMedGoogle Scholar
  • 9 Herman AG, Claeys M, Moncada S, Vane JR. Biosynthesis of prostacyclin (PGI2) and 12 1-hydroxy 5,8,10,14 eichosatetraenoic acid (HETE) by pericardium, pleura and peritoneum and aorta of rabbit. Prostaglandins 1979; 18: 439-444
    CrossrefPubMedGoogle Scholar
  • 10 Coene MC, Solheid C, Claeys M, Herman AG. Prostaglandin production by cultured mesothelial cells. Arch Int Pharmacodyn 1981; 249: 316-318
    PubMedGoogle Scholar