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Thromb Haemost 1984; 51(02): 254-256
DOI: 10.1055/s-0038-1661070
Original Article
Schattauer GmbH Stuttgart

High and Low Affinity Heparin Compared with Unfractionated Heparin as Antithrombotic Drugs

R E Merton
The National Institute for Biological Standards and Control, Holly Hill, Hampstead, London, U. K.
,
D P Thomas
The National Institute for Biological Standards and Control, Holly Hill, Hampstead, London, U. K.
,
S J Havercroft
The National Institute for Biological Standards and Control, Holly Hill, Hampstead, London, U. K.
,
T W Barrowcliffe
The National Institute for Biological Standards and Control, Holly Hill, Hampstead, London, U. K.
,
U Lindahl
*   The Department of Medical and Physiological Chemistry, Swedish University of Agricultural Sciences, The Biomedical Centre, Uppsala, Sweden
› Author Affiliations
Further Information

Publication History

Received 10 January 1984

Accepted 20 February 1984

Publication Date:
19 July 2018 (online)

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Summary

A preparation of heparin was separated by affinity chromatography into two fractions: one of high (HAH) and the other of low (LAH) affinity to antithrombin III. These two fractions were compared with unfractionated heparin (UFH) by in vitro assay and their ability to impair experimental stasis thrombosis was also examined. Although the in vitro activity of HAH was double that of UFH, HAH was less effective than UFH as an antithrombotic drug; LAH was virtually inactive, both in vitro and in vivo. A mixture of 30 μg/kg of HAH and 50 μg/kg of LAH was as effective in preventing thrombosis as 80 μg/kg of UFH, and was more effective than 40 μg/kg of HAH alone, demonstrating that LAH potentiates the action of HAH in vivo.

Keywords

High affinity heparin - Low affinity heparin - Venous thrombosis
 

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