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Thromb Haemost 1984; 51(01): 115-118
DOI: 10.1055/s-0038-1661035
Original Article
Schattauer GmbH Stuttgart

A Comparison of Spontaneous Platelet Aggregation in Whole Blood with Platelet Rich Plasma: Additional Evidence for the Role of ADP

A R Saniabadi
The University Department of Medicine, Royal Infirmary, Glasgow, and the Bioengineering Unit, University of Strathclyde, Glasgow, U. K.
,
G D O Lowe
The University Department of Medicine, Royal Infirmary, Glasgow, and the Bioengineering Unit, University of Strathclyde, Glasgow, U. K.
,
J C Barbenel
The University Department of Medicine, Royal Infirmary, Glasgow, and the Bioengineering Unit, University of Strathclyde, Glasgow, U. K.
,
C D Forbes
The University Department of Medicine, Royal Infirmary, Glasgow, and the Bioengineering Unit, University of Strathclyde, Glasgow, U. K.
› Author Affiliations
Further Information

Publication History

Received 04 October 1983

Accepted 09 December 1983

Publication Date:
19 July 2018 (online)

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Summary

ADP, generated from red blood cells is believed to be responsible for the spontaneous aggregation of platelets in whole blood. This notion is based mainly on the use of enzymes which remove ADP. We have studied spontaneous platelet aggregation in whole blood and autologous platelet rich plasma obtained from 12 healthy male and female volunteers. Platelet aggregation was quantitated by measuring the fall in the number of single platelets counted using a whole blood platelet counter (Ultra Flo 100). In a rotating tube model, the mean fall in the number of platelets due to spontaneous aggregation was 56% in whole blood but, only 3% in platelet rich plasma prepared from the same blood samples. Spontaneous platelet aggregation in whole blood was unaffected by apyrase grade I, but was reduced to 15% by apyrase grade II, to 38% by creatine phosphokinase/creatine phosphate and to 9% by pyruvate kinase/phosphoenolpyruvate. The results of this study provide additional evidence that ADP generated in whole blood triggers the spontaneous aggregation of platelets.

Key Words

Red blood cells - ADP - Spontaneous platelet aggregation
 

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