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Thromb Haemost 1984; 51(01): 032-036
DOI: 10.1055/s-0038-1661013
Original Article
Schattauer GmbH Stuttgart

The Effect of Albumin Bound Polyunsaturated Fatty Acids on Human Platelets

Stein Holme
The Department of Medicine, Institute for Clinical Medicine, University of Tromsø, Tromsø, Norway
,
Jan H Brox
The Department of Medicine, Institute for Clinical Medicine, University of Tromsø, Tromsø, Norway
,
Hedvig Krane
The Department of Medicine, Institute for Clinical Medicine, University of Tromsø, Tromsø, Norway
,
Arne Nordøy
The Department of Medicine, Institute for Clinical Medicine, University of Tromsø, Tromsø, Norway
› Author Affiliations
Further Information

Publication History

Received 24 May 1983

Accepted 09 November 1983

Publication Date:
19 July 2018 (online)

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Summary

Platelet rich plasma (PRP) was incubated at 22–24° C for 24 hr with various polyunsaturated fatty acids (PUFA), bound to albumin, to determine whether changes in the FA composition of the platelet phospholipids might induce changes in platelet behavior and in platelet structural integrity. The PUFA tested were linoleic (18:2 w6)*, linolenic (18:3 w3), dihomo-gamma linolenic (20:3 w6), arachidonic (20:4 w6) and eicosapentaenoic acids (20:5 w3). Only PRP incubated with 20:3 w6 and 20:5 w3 incorporated significant amounts of these FA into the platelet phospholipids and showed inhibition of aggregation and TxB2 production with collagen. However, the inhibition was not due to the incorporation of these FA’s into the phospholipids as observed by washing and resuspension of the FA incorporated platelets in control plasma, or to any change in structural integrity. It is suggested that the effect of 20:3 w6 and 20:5 w3 on aggregation is due to metabolites of these FA’s released from the platelets.

Key Words

Platelets - Polyunsaturated fatty acids - Aggregation - Structural integrity
 

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