Thromb Haemost 1997; 78(02): 960-961
DOI: 10.1055/s-0038-1657661
Letter to the Editor
Schattauer GmbH Stuttgart

Multiplex PCR-mediated Site-directed Mutagenesis for One-step Determination of Factor V Leiden and G20210A Transition of the Prothrombin Gene

L Ripoll
The Laboratoire d’Hématologie biologique et moléculaire, Hôpital Lariboisière, Paris, France
,
D Paulin
The Laboratoire d’Hématologie biologique et moléculaire, Hôpital Lariboisière, Paris, France
,
S Thomas
The Laboratoire d’Hématologie biologique et moléculaire, Hôpital Lariboisière, Paris, France
,
L O Drouet
The Laboratoire d’Hématologie biologique et moléculaire, Hôpital Lariboisière, Paris, France
› Author Affiliations
Further Information

Publication History

Received 18 1997

Accepted after revision 02 April 1997

Publication Date:
30 July 2018 (online)

 
  • References

  • 1 Bertina RM, Koeleman BPC, Koster T, Rosendaal FT, Dirven RJ, de RondeH, van derVelden PP, Reitsma PH. Mutation in blood coagulation factor V associated with resistance to activated protein C. Nature 1994; 369: 64-67
  • 2 Koster T, Rosendaal FR, de RondeH, Briët E, Vandenbroucke JP, Bertina RM. Venous thrombosis due to poor anticoagulant response to activated protein C: Leiden thrombophilia study. Lancet 1993; 342: 1503-1506
  • 3 Koeleman BPC, Reitsma PH, Allaart CF, Bertina RM. Activated protein C resistance as an additional risk factor for thrombosis in protein C-deficient families. Blood 1994; 84: 1031-1035
  • 4 Poort SR, Rosendaal FR, Reitsma PH, Bertina RM. A common genetic variation in the 3’-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. Blood 1995; 88: 3698-3703
  • 5 Haliassos A, Chomel JC, Tesson L, Baudis M, Kruh J, Kaplan JC, Kitzis A. Modification of enzymatically amplified DNA for the detection of point mutation. Nucl Acids Res 1989; 17: 3606
  • 6 Walsh PS, Metzger DA, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. 1991 Biotechniques 10: 506-513
  • 7 Rabes J-P, Trossaert M, Connard J, Samama M, Giraudet P, Boileau C. Single point mutation at the Arg 506 of the factor V associated with APC resistance and the venous thromboembolism: improved detection by PCR-mediated site-directed mutagenesis. Thromb Haemost 1995; 75: 1379-1387
  • 8 Degen SJF, Davie EW. Nucleotide sequence of the gene for human prothrombin. Biochemistry 1987; 26: 6165-6177