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Thromb Haemost 1997; 78(02): 897-901
DOI: 10.1055/s-0038-1657649
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Schattauer GmbH Stuttgart

Thrombospondin Induces Dimerization of Membrane-Bound, but not Soluble CD36

L Daviet
1   The INSERM Unit 331, Faculty of Medicine RTH Laënnec, Lyon, France
,
E Malvoisin
2   INSERM Unit 404, Institut Pasteur de Lyon, Lyon, France
,
T F Wild
2   INSERM Unit 404, Institut Pasteur de Lyon, Lyon, France
,
J L McGregor
3   Stanford Medical School, Division of Hematology (S1 61), Stanford, USA
› Author Affiliations
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Publication History

Received 10 1996

Accepted after revision 19 March 1997

Publication Date:
12 July 2018 (online)

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Summary

CD36 is a cell surface receptor that has been shown to interact with a large variety of ligands including thrombospondin, collagen, Plasmodium falciparum-infected erythrocytes, apoptotic neutrophils, modified low density lipoproteins, anionic phospholipids and long chain fatty acids. A number of these CD36 ligands elicit the transduction of intracellular signals involved in cell activation and internalization of bound ligands. The engagement of CD36 possibly activates three cytosolic protein tyrosine kinases that are presumably associated with the C- terminal cytoplasmic tail of CD36. However, the mechanisms by which CD36 functions in ligand binding and signal transduction are poorly understood. In the present study, a membrane-bound and a truncated soluble form of CD36 were expressed in HeLa cells and analyzed by velocity-gradient centrifugation and chemical cross-linking. We show that membrane CD36 exists predominantly as a monomer but a homo- dimeric form is also found. In contrast, soluble CD36 sedimented in sucrose gradient as a monomer. However, when incubated with thrombospondin, the membrane form of CD36 predominantly sedimented as a dimer whereas soluble CD36 was monomeric. This study shows that thrombospondin has the ability to induce dimerization of CD36 and may be implicated in the signal transduction capacity of this adhesion molecule.

 

  • References

  • 1 Asch AS, Barnwell J, Silverstein RJ, Nachmann RL. Isolation of the thrombospondin membrane receptor. J Clin Invest 1987; 79: 1054-1061
    CrossrefPubMedGoogle Scholar
  • 2 Tandon NN, Kralisz U, Jamieson GA. Identification of glycoprotein IV (CD36) as a primary receptor for platelet-collagen adhesion. J Biol Chem 1989; 264: 7576-7583
    PubMedGoogle Scholar
  • 3 Oquendo P, Hundt E, Lawler J, Seed B. CD36 directly mediates cyto-adher- ence of Plasmodium falciparum-parasitized erythrocytes. Cell 1989; 58: 095-101
    CrossrefPubMedGoogle Scholar
  • 4 Savill J, Hogg N, Ren Y, Haslett C. Thrombospondin cooperates with CD36 and the vitronectin receptor in macrophage recognition of neutrophils undergoing apoptosis. J Clin Invest 1992; 90: 1513-1522
    CrossrefPubMedGoogle Scholar
  • 5 Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem 1993; 268: 11811-11816
    PubMedGoogle Scholar
  • 6 Abumrad NA, El-Maghrabi MR, Amri E, Lopez E, Grimaldi PA. Cloning of a rat adipocyte membrane protein implicated in binding or transport of long-chain fatty acids that is induced during preadipocyte differentiation- homology with human CD36. J Biol Chem 1993; 268: 17665-17668
    PubMedGoogle Scholar
  • 7 Aiken ML, Ginsberg MH, Byers-Ward V, Plow EF. Effects of OKM5, a monoclonal antibody to glycoprotein IV, on platelet aggregation and thrombospondin surface expression. Blood 1990; 76: 2501-2509
    PubMedGoogle Scholar
  • 8 Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ocken-house CF, Jamieson GA, Malavasi F. Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07. Blood 1993; 82: 3637-3647
    PubMedGoogle Scholar
  • 9 Ockenhouse CF, Magowan C, Chulay JD. Activation of monocytes and platelets by monoclonal antibodies or malaria-infected erythrocytes binding to the CD36 surface receptor in vitro. J Clin Invest 1989; 84: 468-475
    CrossrefPubMedGoogle Scholar
  • 10 Schüiiepp BJ, Pfister H, Clemetson KJ, Silverstein RL, Jungi TW. CD36-mediated signal transduction in human monocytes by anti-CD36 antibodies but not by anti-thrombospondin antibodies recognizing cell membrane-bound thrombospondin. Biochem Biophys Res Commun 1991; 175: 263-270
    CrossrefPubMedGoogle Scholar
  • 11 Huang MM, Bolen JB, Barnwell JW, Shattil SJ, Brugge JS. Membrane glycoprotein IV (CD36) is physically associated with the Fyn, Lyn, and Yes protein-tyrosine kinases in human platelets. Proc Natl Acad Sci USA 1991; 88: 7844-7848
    CrossrefPubMedGoogle Scholar
  • 12 Pearce SFA, Wu J, Silverstein RL. A carboxy terminal truncation mutant of CD36 is secreted and binds thrombospondin: evidence for a single transmembrane domain. Blood 1994; 84: 384-389
    PubMedGoogle Scholar
  • 13 Bolen JB. Signal transduction by the src family of tyrosine kinases in hematopoietic cells. Cell Growth Differ 1991; 02: 409-414
    PubMedGoogle Scholar
  • 14 McGregor JL, Catimel B, Parmentier S, Clezardin P, Dechavanne M, Leung LLK. Rapid purification and partial characterization of human platelet glycoprotein Illb-Interaction with thrombospondin and its role in platelet aggregation. J Biol Chem 1989; 264: 501-506
    PubMedGoogle Scholar
  • 15 Daviet L, Buckland R, Puente NavazoMD, McGregor L. Identification of an immunodominant, functional domain on human CD36 using human- mouse chimeric proteins and homologue replacement mutagenesis. Biochem J 1995; 305: 221-224
    CrossrefPubMedGoogle Scholar
  • 16 Parmentier S, McGregor L, Catimel B, Leung LKK, McGregor JL. Inhibition of platelet functions by a monoclonal antibody (LYP 20) directed against a granule membrane glycoprotein (GMP140/PADGEM). Blood 1991; 77: 1734-1739
    PubMedGoogle Scholar
  • 17 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970; 227: 680-685
    CrossrefPubMedGoogle Scholar
  • 18 Malvoisin E, Wild TF. Analysis of the human immunodeficiency virus type 1 envelope protein interaction with the CD4 host cell receptor. J Gen Virol 1994; 75: 839-847
    CrossrefPubMedGoogle Scholar
  • 19 Asch AS, Silbiger S, Heimer E, Nachman RL. Thrombospondin sequence motif (CSVTCG) is responsible for CD36 binding. Biochem Biophys Res Commun 1992; 182: 1208-1217
    CrossrefPubMedGoogle Scholar
  • 20 Silverstein RL, Baird M, Lo SK, Yesner LM. Sense and antisense cDNA transfection of CD36 (glycoprotein IV) in melanoma cells. Role of CD36 as a thrombospondin receptor. J Biol Chem 1992; 267: 16607-16612
    PubMedGoogle Scholar
  • 21 Yarden Y, Schlessinger J. Self-phosphorylation of epidermal growth factor receptor: evidence for a model of intermolecular allosteric activation. Biochemistry 1987; 26: 1434-1442
    CrossrefPubMedGoogle Scholar
  • 22 Reilly PL, Woska Jr JR, Jeanfavre DD, McNally E, Rothlein R, Bormann BJ. The native structure of Intercellular Adhesion Molecule-1 (ICAM-1) is a dimer-Correlation with binding to LFA-1. J Immunol 1995; 155: 529-532
    PubMedGoogle Scholar
  • 23 Bormann BJ, Engelman DM. Intramembrane helix-helix association in oligomerization and transmembrane signaling. Annu Rev Biophys Biomo- lecular Struct 1992; 21: 223-242
    PubMedGoogle Scholar
  • 24 Greenlund AC, Schreiber RD, Goeddel DV, Pennica D. Interferon-g induces receptor dimerization in solution and on cells. Biochemistry 1993; 268: 18103-18110
    PubMedGoogle Scholar
  • 25 Pennica D, Lam VT, Weber RF, Kohr WJ, Basa LJ, Spellman MW, Ashkenazi A, Shire SJ, Goeddel DV. Biochemical characterization of the extracellular domain of the 75-kilodalton tumor necrosis factor receptor. Biochemistry 1993; 323: 3131-3138
    PubMedGoogle Scholar
  • 26 Li WX, Howard RJ, Leung LLK. Identification of SVTCG in thrombospondin as the conformation-dependant, high affinity binding site for its receptor, CD36. J Biol Chem 1993; 268: 16179-16184
    PubMedGoogle Scholar
  • 27 Pearce SFA, Wu J, Silverstein RL. Recombinant GST/CD36 fusion proteins define a thrombospondin binding domain. J Biol Chem 1995; 270: 2981-2986
    CrossrefPubMedGoogle Scholar
  • 28 Ibrahimi A, Sfeir Z, Magharaie H, Amri E-Z, Grimaldi P, Abumrad NA. Expression of the CD36 homolog (FAT) in fibroblast cells; Effects on fatty acid transport. Proc Natl Acad Sci USA 1996; 93: 2646-2651
    CrossrefPubMedGoogle Scholar
  • 29 Rhinehart-Jones T, Greenwalt DE. A detergent-sensitive 113-kDa con- former/complex of CD36 exists on the platelet surface. Arch Biochem Biophys 1996; 326: 115-118
    CrossrefPubMedGoogle Scholar