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Thromb Haemost 1997; 78(01): 543-547
DOI: 10.1055/s-0038-1657585
Transgenic production of hemostasis proteins
Schattauer GmbH Stuttgart

The Past, Present and Future of Transgenic Bioreactors

William N Drohan
J. Holland Laboratory, American Red Cross, Rockville, MD, USA
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Publication History

Publication Date:
12 July 2018 (online)

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  • References

  • 1 Palmiter RD, Brinster RL, Hammer RE, Trumbauer ME, Rosenfeld MG, Birnberg NC, Evans RM. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 1982; 300: 611-615
    CrossrefPubMedGoogle Scholar
  • 2 Gordon JW, Scangos GA, Plotkin DJ, Barbosa JA, Ruddle FH. Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci USA 1980; 77: 7380-7384
    CrossrefPubMedGoogle Scholar
  • 3 Gordon JW, Ruddle FH. Integration and stable germ line transmission of genes injected into mouse pronuclei. Science 1981; 214: 1244-1246
    CrossrefPubMedGoogle Scholar
  • 4 Harbers K, Jahner D, Jaenisch R. Microinjection of cloned retroviral genomes into mouse zygotes: Integration and expression in the animal. Nature 1981; 293: 540-542
    CrossrefPubMedGoogle Scholar
  • 5 Wagner EF, Stewart TA, Mintz B. The human β-globin gene and a functional viral thymidine kinase gene in developing mice. Proc Natl Acad Sci USA 1981; 78: 5016-5020
    CrossrefPubMedGoogle Scholar
  • 6 Wagner TE, Hoppe PC, Jollick JD, Scholl DR, Hodinka RL, Gault JB. Microinjection of a rabbit β-globin gene into zygotes and its subsequent expression in adult mice and their offspring. Proc Natl Acad Sci USA 1981; 78: 6376-6380
    CrossrefPubMedGoogle Scholar
  • 7 Constantini F, Lacy E. Introduction of a rabbit β-globin gene into the mouse germ line. Nature 1981; 294: 92-94
    CrossrefPubMedGoogle Scholar
  • 8 Brinster RL, Chen Y, Trumbauer M, Senear AW, Warren R, Palmiter RD. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell 1981; 27: 223-231
    CrossrefPubMedGoogle Scholar
  • 9 Brinster RL, Ritchie KA, Hammer RE, O’Brien RL, Arp B, Storb U. Expression of a microinjected immunoglobulin in the spleen of transgenic mice. Nature 1983; 306: 332-336
    CrossrefPubMedGoogle Scholar
  • 10 Swift GH, Hammer RE, MacDonald RJ, Brinster RL. Tissue-specific expression of the rat pancreatic elastase I gene in transgenic mice. Cell 1984; 38: 639-646
    CrossrefPubMedGoogle Scholar
  • 11 Wall RJ, Pursel VG, Hammer RE, Brinster RL. Development of porcine ova that were centrifuged to permit visualization of pronuclei and nuclei. Biol Reprod 1985; 32: 645-651
    CrossrefPubMedGoogle Scholar
  • 12 Hammer RE, Pursel VG, Rexroad CE, Wall RJ, Bolt DJ, Ebert KM, Palmiter RD, Brinster RL. Production of transgenic rabbits, sheep and pigs by microinjection. Nature 1985; 315: 680-683
    CrossrefPubMedGoogle Scholar
  • 13 Brem G, Brenig B, Goodman HM, Selden RC, Graf F, Kruff B, Springmann K, Hondele J, Meyer J, Winnacker EL, Kräuβlich H. Production of transgenic mice, rabbits and pigs by microinjection into pronuclei. Zuchthygiene 1985; 20: 251-252
    CrossrefPubMedGoogle Scholar
  • 14 Bühler TA, Bruyere T, Went DF, Stranzinger G, Burki K. Rabbit β-casein promoter directs secretion of human interleukin-2 into the milk of transgenic rabbits. Bio/Technology 1990; 8: 140-142
    CrossrefPubMedGoogle Scholar
  • 15 Ebert KM, Selgrath JP, DiTullio P, Denman J, Smith TE, Memon MA, Schindler JE, Monastersky GM, Vitale JA, Gordon K. Transgenic production of a variant of human tissue-type plasminogen activator in the milk: Generation of transgenic goats and analysis of expression. Bio/Technology 1991; 09: 835-838
    CrossrefPubMedGoogle Scholar
  • 16 Krimpenfort P, Rademakers A, Eyestone W, van der Schans A, van den Broek S, Kooiman P, Kootwijk E, Platenburg G, Pieper F, Strijker R, de Boer H. Generation of transgenic dairy cattle using 'in vitro’ embryo production. Bio/Technology 1991; 09: 844-847
    CrossrefPubMedGoogle Scholar
  • 17 Hill KG, Curry J, DeMayo FJ, Jones-Diller K, Slapak JR, Bondioli KR. Production of transgenic cattle by pronuclear injection. Theriogenology 1992; 37: 222
    CrossrefPubMedGoogle Scholar
  • 18 Campbell SM, Rosen JM, Hennighausen L, Strech-Jurk U, Sippel AE. Comparison of the whey acidic protein genes of the rat and mouse. Nucl Acids Res 1984; 12: 8685-8697
    CrossrefPubMedGoogle Scholar
  • 19 Ali S, Clark AJ. Characterization of the gene encoding ovine beta-lactoglobulin. Similarity to the gene for retinol binding protein and other secretory proteins. J Mol Biol 1988; 199: 415-426
    CrossrefPubMedGoogle Scholar
  • 20 Andres AC, Schoenenberger CA, Groner B, Hennighausen L, LeMeur M, Gerlinger P. Ha-ras oncogene expression directed by a milk protein gene promoter: Tissue specificity, hormonal regulation, and tumor induction in transgenic mice. Proc Natl Acad Sci 1987; 84: 1299-1303
    CrossrefPubMedGoogle Scholar
  • 21 Simons JP, McClenaghan M, Clark AJ. Alteration of the quality of milk by expression of sheep β-lactoglobulin in transgenic mice. Nature 1987; 328: 530-532
    CrossrefPubMedGoogle Scholar
  • 22 Gordon KI, Lee E, Vitale JA, Smith AE, Westphal H, Hennighausen L. Production of human tissue plasminogen activator in transgenic mouse milk. Bio/Technology 1987; 05: 1183-1187
    CrossrefPubMedGoogle Scholar
  • 23 Pittius CW, Hennighausen L, Lee E, Westphal H, Nicols E, Vitale J, Gordon K. A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice. Proc Natl Acad Sci 1988; 85: 5874-5878
    CrossrefPubMedGoogle Scholar
  • 24 Clark AJ, Bessos H, Bishop JO, Brown P, Harris S, Lathe R, McClenaghan M, Prowse C, Simons JP, Whitelaw CBA, Wilmut I. Expression of human anti-hemophilic factor IX in the milk of transgenic sheep. Bio/Technology 1989; 07: 487-492
    CrossrefPubMedGoogle Scholar
  • 25 Grosveld R, van Assendelft-Blom G, Greaves DR, Kolias G. Position-independent high-level expression of the human β-globingene in transgenic mice. Cell 1987; 51: 975-985
    CrossrefPubMedGoogle Scholar
  • 26 Hanscombe O, Vidal M, Kaeda J, Luzzatto L, Greaves DR, Grosveld F. High-level erythroid-specific expression of the human α-globin gene in transgenic mice and the production of human hemoglobin in murine erythrocytes. Genes Dev 1989; 03: 1572-1581
    CrossrefPubMedGoogle Scholar
  • 27 Behringer RR, Ryan TM, Reilly MP, Asakura T, Palmiter RD, Brinster RL, Townes TM. Synthesis of fimctional human hemoglobin in transgenic mice. Science 1989; 245: 971-973
    CrossrefPubMedGoogle Scholar
  • 28 Carver AS, Dalrymple MA, Wright G, Cottom DS, Reeves DB, Gibson YH, Keenan JL, Barass JD, Scott AR, Colman A, Garner I. Transgenic livestock as bioreactors: Stable expression of human alpha-1-antitrypsin by a flock of sheep. Bio/Technology 1993; 11: 1263-1269
    CrossrefPubMedGoogle Scholar
  • 29 Pursel VG, Pinkert CA, Miller KF, Bolt DJ, Campbell RG, Palmiter RD, Brinster RL, Hammer RE. Genetic engineering of livestock. Science 1989; 244: 1281-1287
    CrossrefPubMedGoogle Scholar
  • 30 Van Cott KE, Lubon H, Russell CG, Butler SP, Gwazdauskas FC, Knight J, Drohan NW, Velander WH. Phenotypic and genotypic stability of multiple lines of transgenic pigs expressing recombinant Protein C. Transgenic Res. in press
    Google Scholar
  • 31 Denman J, Hayes M, O’Day C, Edmunds T, Bartlett C, Hirani S, Ebert KM, Gordon K, McPherson JM. Transgenic expression of a variant of human tissue-type plasminogen activator in goat milk: purification and characterization of the recombinant enzyme. Bio/Technology 1991; 09: 839-843
    CrossrefPubMedGoogle Scholar
  • 32 Shani M, Barash I, Nathan M, Ricca G, Searfoss GH, Dekel I, Faerman A, Givol D, Hurwitz DR. Expression of human serum albumin in the milk of transgenic mice. Transgenic Res 1992; 01: 195-208
    CrossrefPubMedGoogle Scholar
  • 33 Barash I, Faerman A, Ratovitsky T, Puzis R, Nathan M, Hurwitz DR, Shani M. Ectopic expression of β-lactoglobulin/human serum albumin fusion genes in transgenic mice: Hormonal regulation and in situ localization. Transgenic Res 1994; 03: 141-151
    CrossrefPubMedGoogle Scholar
  • 34 Yuli F, Harold G, Wallace R, Cowper A, Percy J, Cottingham I, Clark A. Fixing human factor IX (fIX): correction of a cryptic RNA splice enables the production of biologically active fIX in the mammaiy gland of transgenic mice. Proc Natl Acad Sci USA 1995; 92: 10899-10903
    CrossrefPubMedGoogle Scholar
  • 35 Swanson ME, Martin MJ, O’Donnell K, Hoover K, Lago W, Huntress V, Parsons CT, Pinkert CA, Pilder S, Logan JS. Production of functional human hemoglobin in transgenic swine. Bio/Technology 1992; 10: 557-559
    CrossrefPubMedGoogle Scholar
  • 36 Sharma A, Matin MJ, Okabe JF, Truglio RA, Dhanjal NK, Logan JS, Kumar R. An isologous promoter permits high level expression of human hemoglobin in transgenic swine. Bio/Technology 1994; 12: 55-59
    CrossrefPubMedGoogle Scholar
  • 37 Rao MJ, Schneider K, Chait BT, Chao TL, Keller H, Anderson S, Manjula BN, Kumar R, Achaiya AS. Recombinant hemoglobin A produced in transgenic swine: structural equivalence with human hemoglobin A. Art Cells Blood Subs and Immob Biotech 1994; 22: 695-700
    PubMedGoogle Scholar
  • 38 Sharma A, Khoury-Christianson AM, White SP, Dhanjal NK, Huang W, Paulhiac C, Friedman EF, Manjula BN, Kumar R. High-efficiency synthesis of human α-endorphin and magainin in the erythrocytes of transgenic mice: a production system for therapeutic peptides. Proc Natl Acad Sci USA 1994; 91: 9337-9341
    CrossrefPubMedGoogle Scholar
  • 39 Wright G, Carver A, Cottom D, Reeves D, Scott A, Simons P, Wilmut I, Gamer I, Colman A. High level expression of active human alpha-1-antitrypsin in the milk of transgenic sheep. Bio/Technology 1991; 09: 830-834
    CrossrefPubMedGoogle Scholar
  • 40 Colman A, Gamer I. The transgenic mammary gland as a bioreactor: Expectations and realizations. In Proceedings of the 1995 Miami Bio/Technology Winter Symposium, Advances in Gene Technology, IRL Press at Oxford University Press, Miami Bio/Technology Short Reports. 1995. 6. 107
    Google Scholar
  • 41 Prunkard D, Cottingham I, Gamer I, Bruce S, Dalrymple M, Lasser G, Bishop P, Foster D. High-level expression of recombinant human fibrinogen in the milk of transgenic mice. Nature Biotech 1996; 14: 867-871
    CrossrefPubMedGoogle Scholar
  • 42 Hurwitz DR, Nathan M, Barash I, Ilan N, Shani M. Specific combinations of human serum albumin introns direct high level expression of albumin in transfected COS cells and in the milk of transgenic mice. Transgenic Res 1994; 03: 365-375
    CrossrefPubMedGoogle Scholar
  • 43 Carver A, Wright G, Cottom D, Cooper J, Dalrymple M, Temperley S, Udell M, Reeves D, Percy J, Scott A, Barrass D, Gibson Y, Jeffrey Y, Samuel C, Colman A, Gamer I. Expression of human α1 antitrypsin in transgenic sheep. Cytotechnology 1992; 09: 77-84
    CrossrefPubMedGoogle Scholar
  • 44 James DC, Freedman RB, Hoare M, Ogonah OW, Rooney BC, Larionov OA, Dobrovolsky VN, Lagutin OV, Jenkins N. Nlycosylation of recombinant human interferon-γ produced in different animal expression systems. Bio/Technology 1995; 13: 592-596
    CrossrefPubMedGoogle Scholar
  • 45 James DC, Goldman MH, Hoare M, Jenkins N, Oliver RWA, Green BN, Freedman RB. Posttranslational processing of recombinant human interferon-γ in animal expression systems. Prot Sci 1996; 05: 331-340
    PubMedGoogle Scholar
  • 46 Strömqvist M, Törnell J, Edlund M, Edlund A, Johansson T, Lindgren K, Lundberg L, Hansson L. Recombinant human bile salt-stimulated lipase: An example of defective O-glycosylation of a protein produced in milk of transgenic mice. Transgenic Res 1996; 05: 475-485
    CrossrefPubMedGoogle Scholar
  • 47 Wei Y, Yarns S, Greenberg NM, Whitsett J, Rosen JM. Production of human surfactant protein C in milk of transgenic mice. Transgenic Res 1995; 04: 232-240
    CrossrefPubMedGoogle Scholar
  • 48 Langford GA, Cozzi E, Yannoutsos N, Lancaster R, Elsome K, Chen P, White DJG. Production of pigs transgenic for human regulators of complement activation using YAC technology. Transplant Proc 1996; 28: 862-863
    PubMedGoogle Scholar
  • 49 Drews R, Paleyanda RK, Lee TK, Chang RR, Rehemtulla A, Kaufman RJ, Drohan WN, Lubon H. Proteolytic maturation of Protein C upon engineering the mouse mammary gland to express furin. Proc Natl Acad Sci USA 1995; 92: 10462-10466
    CrossrefPubMedGoogle Scholar
  • 50 Prieto PA, Mukerji P, Kelder B, Emey R, Gonzalez D, Yun JS, Smith DF, Moremen KW, Nardelli C, Pierce M, Li Y, Chen X, Wagner TE, Cummings RD, Kopchick JJ. Remodeling of mouse milk glycoconjugates by transgenic expression of a human glycosyltransferase. J Biol Chem 1995; 49: 29515-29519
    PubMedGoogle Scholar
  • 51 Koike C, Hayashi S, Yokoyama I, Yamakawa H, Negita M, Takagi H. Converting α-Gal epitope of pig into H antigen. Transplant Proc 1996; 28: 553
    PubMedGoogle Scholar
  • 52 Fodor WL, William GL, Matis LA, Madri JA, Rollins SA, Knight JW, Velander W, Squinto SP. Expression of a functional human complement inhibitor in a transgenic pig as a model for the prevention of xenogeneic hyperacute organ rejection. Proc Natl Acad Sci USA 1994; 91: 11153-11157
    CrossrefPubMedGoogle Scholar
  • 53 Cozzi E, Langord GA, Wright L, Pino-Chávez G, Levy A, Chatteijee M, Yannoutsos N, Miller N, Lancaster R, White DJG. Longitudinal analysis of the expression of human decay accelerating factor of peripheral blood mononuclear cells and in the plasma of transgenic pigs. Transplant Proc 1996; 28: 860-861
    PubMedGoogle Scholar
  • 54 Kroshus TJ, Bolman III RM, Dalmasso AP, Rollins SA, Guilmette ER, Williams BL, Squinto SP, Fodor WL. Expression of human CD59 in transgenic pig organs enhances organ survival in an ex vivo xenogeneic perfusion model. Transplantation 1996; 61: 1513-1521
    CrossrefPubMedGoogle Scholar
  • 55 Byme G, McCuriy K, Martin M, Platt J, Logan J. Development and analysis of transgenic pigs expressing the human complement regulatory proteins CD59 and DAF. Transplant Proc 1996; 28: 759
    PubMedGoogle Scholar
  • 56 Archibald AL, McClenaghan M, Homsey V, Simons JP, Clark AJ. High level expression of biologically active human α1-antitrypsin in the milk of transgenic mice. Proc Natl Acad Sci 1990; 87: 5178-5182
    CrossrefPubMedGoogle Scholar
  • 57 Thompson PB. Genetically modified animals: Ethical issues. J Anim Sci 1993; 71 (Suppl. 03) 51-56
    PubMedGoogle Scholar