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Thromb Haemost 1997; 77(03): 555-561
DOI: 10.1055/s-0038-1656005
Platelets
Schattauer GmbH Stuttgart

ADP-mimicking Platelet Aggregation Caused by Rugosin E, an Ellagitannin Isolated from Rosa rugosa Thunb

Che-Ming Teng
1   The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan
,
Ya-Fei Kang
1   The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan
,
Ya-Ling Chang
1   The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan
,
Feng-Nien Ko
1   The Pharmacological Institute, College of Medicine, National Taiwan University, Taipei, Taiwan
,
Shu-Chen Yang
2   The School of Pharmacy, Taipei Medical College, Taipei, Taiwan
,
Feng-Lin Hsu
2   The School of Pharmacy, Taipei Medical College, Taipei, Taiwan
› Author Affiliations
Further Information

Publication History

Received 14 May 1996

Accepted after revision 04 November 1996

Publication Date:
11 July 2018 (online)

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Summary

Among the nine ellagitannins, rugosin E was the most potent platelet aggregating agent with an EC50 of 1.5 ± 0.1 µM in rabbit platelets and 3.2 ±0.1 µM in human platelets. The aggregations caused by rugosin E and ADP were inhibited by EGTA, PGE1, mepacrine, sodium nitroprusside and neomycin, but not by indomethacin, verapamil, TMB-8, BN52021 and GR32191B. Rugosin E-induced thromboxane formation was suppressed by indomethacin, EGTA, PGE,, verapamil, mepacrine, TMB-8 and neomycin. ADP-scavenging agents, such as CP/CPK and apyrase inhibited concentration-dependently ADP (20 εM)-, but not rugosin E (5 εM)-induced platelet aggregation. In thrombin (0.1 U/ml)-treated and degranulated platelets, rugosin E and ADP still caused 63.5 ± 3.0% and 61.2 ± 3.5% of platelet aggregation, respectively. Selective ADP receptor antagonists, ATP and FSBA inhibited rugosin E- and ADP-induced platelet aggregations in a concentration-dependent manner. Both rugosin E and ADP did not induce platelet aggregation in ADP (1 mM)-desensitized platelets. In contrast to ADP, rugosin E did not decrease cAMP formation in washed rabbit platelets. Both rugosin E and ADP did not cause phosphoinositide breakdown in [3H]myo-inositol-labeled rabbit platelets. In fura-2/AM- load platelets, both rugosin E and ADP induced increase in intracellular calcium concentration and these responses were inhibited by ATP and PGEj. All these data suggest that rugosin E may be an ADP receptor agonist in rabbit platelets.

 

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