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Thromb Haemost 1997; 77(01): 062-070
DOI: 10.1055/s-0038-1655908
Clinical Studies
Schattauer GmbH Stuttgart

Tissue Factor (TF) and Urokinase Plasminogen Activator Receptor (uPAR) and Bleeding Complications in Leukemic Patients

Chary López-Pedrera
,
Merce Jardí
,
Maria del Mar Malagón
The Servicio de Hematología Hospital Reina Sofia, Córdoba, Spain
,
Julia Inglés-Esteve
1   Institut Recerca Oncològica (IRO), Hospital Duran i Reynals, Barcelona, Spain
,
Gabriel Dorado
The Servicio de Hematología Hospital Reina Sofia, Córdoba, Spain
,
Antonio Torres
The Servicio de Hematología Hospital Reina Sofia, Córdoba, Spain
,
Jordi Félez
1   Institut Recerca Oncològica (IRO), Hospital Duran i Reynals, Barcelona, Spain
,
Francisco Velasco
The Servicio de Hematología Hospital Reina Sofia, Córdoba, Spain
› Author Affiliations
Further Information

Publication History

Received 22 January 1996

Accepted after resubmisssion 09 September 1996

Publication Date:
11 July 2018 (online)

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Summary

Tissue factor (TF) and urokinase receptor (uPAR) are key cellular receptors triggering, respectively, coagulation and fibrinolysis. Bleeding complications among leukemic patients have been related to an abnormal expression of TF by blast cells and/or to an abnormal fibrinolytic response. In this study the expression of TF and uPAR has been assessed in 18 acute non-lymphoblastic and 8 lymphoblastic leukemic blast cells using several methodological approaches. TF mRNA was evaluated by in situ hybridization and TF and uPAR antigen were evaluated immunologically in cell lysates and on the cell surface by flow cytometry. In addition, TF-procoagulant activity was measured in coagulation-based assays. The reliability of these methods was corroborated in six leukemic cell lines of different lineages and states of maturation. Disseminated intravascular coagulation was detected in two M3 leukemia patients whose blast cells expressed high amounts of TF. Hyperfibrinolysis was detected in one M1 and two M2 patients, whose blast cells displayed a high content of uPAR antigen, but no TF. Furthermore, M5 leukemia blast cells expressed both TF and uPAR, although no hemostatic defects or bleeding complications were detected in these patients. Taken together, although a limited number of patients was included in this study, these data suggest that in leukemia patients exhibiting bleeding, either TF or uPAR are expressed by their blast cells. However, the presence of these receptors does not necessarily imply the existence of a hemostatic disorder.

 

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