Download PDF
Thromb Haemost 1981; 46(04): 673-675
DOI: 10.1055/s-0038-1653449
Original Article
Schattauer GmbH Stuttgart

In Vitro Effect of Ethanol on ADP and Collagen-Induced Platelet Aggregation

Stig Bengmark
The Department of Surgery, University of Lund, Sweden
,
Olle Elmér
The Department of Surgery, University of Lund, Sweden
,
Göran Göransson
The Department of Surgery, University of Lund, Sweden
,
Evita Zoucas
The Department of Surgery, University of Lund, Sweden
› Author Affiliations
Further Information

Publication History

Received 26 March 1981

Accepted 07 September 1981

Publication Date:
05 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

Platelet aggregation was measured by Born’s method. Plasma from Sprague-Dawley rats and inbred albino rabbits was used. 20% ethanol in 0.9%(w/v) NaCl was added to plasma at final concentrations of 56.5 mM, 85.9 mM 171.5 mM and 343.1 mM or to whole blood at final concentrations of 8.7 mM, 17.4 mM, 52.5 mM, 171.5 mM and 343.1 mM. Experiments using 0.9% NaCl volumes equivalent to the added ethanol volume were also conducted. Dose-response aggregation tests showed that a decrease in ADP-and collagen-induced aggregation existed when ethanol was mixed with rat or rabbit blood prior to the preparation of platelet-rich plasma. There was no effect on ADP-induced platelet aggregation when ethanol was mixed with rat or rabbit plasma. Collagen-induced aggregation was impaired only in rat plasma when ethanol concentration reached 343.1 mM. These results suggested that ethanol modified platelet function possibly via red cells.

Keywords

Ethanol - Platelet rich plasma - Blood - Platelet aggregation
 

  • References

  • 1 Post R, Desforges J. Thrombocytopenia and alcoholism. Ann Intern Med 1968; 68: 1230-1236
    CrossrefPubMedGoogle Scholar
  • 2 Post R, Desofrges J. Thrombocytopenic effect of ethanol infusion. Blood 1968; 31: 344-347
    PubMedGoogle Scholar
  • 3 Haut M, Cowan DH. The effect of ethanol on hemostatic properties of human blood platelets. Am J Med 1974; 56: 22-33
    CrossrefPubMedGoogle Scholar
  • 4 Elmér O, Göransson G, Saku M, Bengmark S. Ethanol-induced microaggregate formation in pig and rabbit blood. An in vitro study Thromb Haemostas 1977; 37: 344-350
    PubMedGoogle Scholar
  • 5 Bengmark S, Göransson G, Idvall J, Zoucas E. Increased bleeding during liver resection in ethanol-intoxicated rats. Thromb Haemostas 1980; 43: 203-207
    PubMedGoogle Scholar
  • 6 Nordensson NG. Klinisk Morfologisk Hematologi. Scandinavian University Books; Stockholm: 1961
    Google Scholar
  • 7 Born BV R. 1962 Quantitative investigation into the aggregation of blood platelets. J Physiol 1962; 162: 67-73
    PubMedGoogle Scholar
  • 8 Zucker MB. Platelet agglutination and vasoconstriction as factors in spontaneous hemostasis in normal, thrombocytopenic, heparinized and hypoprothrombinemic rats. Am J Physiol 1947; 148: 275-282
    PubMedGoogle Scholar
  • 9 Spaet TH. The platelet in hemostasis. Ann N Y Acad Sri 1962; 31: 31-42
    PubMedGoogle Scholar
  • 10 White JG. Combined nephelometric and electron microscope study of the platelet release reaction. Thrombos Diathes Haemorrh 1970; 42 (suppl) 73-91
    PubMedGoogle Scholar
  • 11 Hellem AJ. The adhesiveness of human blood platelets in vitro. Scand J Clin Lab Invest 1960; 12 (suppl) (Suppl. 51) 1-117
    CrossrefPubMedGoogle Scholar
  • 12 Salzman EW. Measurement of platelet adhesiveness. J Lab. Clin. Med 1963; 62: 724-735
    PubMedGoogle Scholar
  • 13 Bowie WE, Owen J, Thompson CA, Didisheim JH. A test of platelet adhesiveness. Mayo Clin Proc 1969; 44: 306-309
    PubMedGoogle Scholar
  • 14 O’Brien JR, Etherington M, Jamieson S, Ainsworth JF. Stressed template bleeding time and other platelet-function tests in myocardial infarction. Lancet 1963; 1: 694-696
    PubMedGoogle Scholar
  • 15 Swank RL. Adhesiveness of platelets and leucocytes during acute exsanguination. Am J Physiol 1962; 202: 261-264
    PubMedGoogle Scholar
  • 16 Dhall DP, Mathesson NA. Platelet aggregate filtration pressure - a method of measuring platelet aggregation on whole blood. Cardiovasc Res 1969; 3: 155-160
    CrossrefPubMedGoogle Scholar
  • 17 Bom BV R, Cross MJ. The aggregation of blood platelets. J Physiol 1963; 168: 178-195
    CrossrefPubMedGoogle Scholar
  • 18 Zweiffler AJ, Sandbar SS. Inhibition of platelet adhesiveness and aggregation by benzylalcohol and phenol. Thrombos Diathes Haemorrh 1969; 21: 362-366
    PubMedGoogle Scholar
  • 19 Davis JW, Phillips PE. The effect of ethanol on human platelet aggregation in vitro. Atherosclerosis 1970; 11: 473-477
    CrossrefPubMedGoogle Scholar
  • 20 Stuart M. Ethanol-inhibited platelet prostaglandin synthesis in vitro. J Stud Alcohol 1979; 40: 1-6
    CrossrefPubMedGoogle Scholar
  • 21 Sinakos S, Caen JP. Platelet aggregation in mammalians (human, rat, rabbit, guinea-pig, horse, dog). A comparative study Thrombos Diathes Haemorrh 1967; 17: 99-101
    PubMedGoogle Scholar
  • 22 Chung-Hsin Tsáo. Rat platelet aggregation by ATP. Am J Pathol 1976; 85: 581-592
    PubMedGoogle Scholar
  • 23 Michel H, Caen JP, Born GV R, Miller R, d’Auriac AnglesG, Meyer P. Relation between the inhibition of aggregation and the concentration of cAMP in human and rat platelets. Br J Haematol 1976; 33: 27-38
    CrossrefPubMedGoogle Scholar
  • 24 Born GV R, Bergqvist D, Arfors KE. Evidence for inhibition of platelet activation in blood by drug effect on erythrocytes. Nature 1976; 259: 223-234
    PubMedGoogle Scholar