Some New Observations on Glycogenolysis in Blood Platelets Studied by Cytochemical Scoring Method

Summary

The breakdown of stainable glycogen in blood platelets was studied by cytochemical scoring technique. Various compounds reacting in different ways with sulfhydryl groups (PCMB, MIA, NEM and ferricyanide) inhibited completely the adrenaline induced glycogenolysis in vitro. The inhibition by PCMB and ferricyanide but not that effected by MIA and NEM could be abolished by addition of reduced glutathione. This substance alone as well as other SH containing compounds (mercaptoethanol and cysteine) produced a marked glycogenolysis at 50 mM concentration. The same glycogenolytic effect was observed with 5 mM cyanide and azide. The degradation of stainable glycogen with all described compounds could be blocked by MIA and fluoride at same concentrations which inhibited the adrenaline induced glycogenolysis. The possible involvement of SH groups in some of these biochemical reactions is discussed.

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