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Thromb Haemost 1969; 22(01): 032-034
DOI: 10.1055/s-0038-1651326
Originalarbeiten-Original Articles-Travaux Originaux
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Note on the Inactivation of Autoprothrombin C with Diisopropylfluorophosphate or Phenylmethanesulfonyl Fluoride[*]

W. H Seegers
1   Department of Physiology and Pharmacology Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.
,
Ewa Marciniak
1   Department of Physiology and Pharmacology Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.
,
L McCoy
1   Department of Physiology and Pharmacology Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.
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Publication History

Publication Date:
10 June 2018 (online)

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Summary

In relatively high concentrations DFP was found to inactivate autoprothrombin C. PMSF also inactivated the enzyme. A useful modification in our assay procedure for autoprothrombin C is described.

* This investigation was supported by a research grant HE-05141-09 from the National Heart Institute, National Institutes of Health, U. S. Public Health Service. We wish to thank Dr. Danica Dabich of our Biochemistry Department for her supplies of material and assistance with some experiments with DFP.


 

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