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Thromb Haemost 1980; 43(02): 108-111
DOI: 10.1055/s-0038-1650028
Original Article
Schattauer GmbH Stuttgart

The Determination of the Heparin Neutralising Capacity of Protamine Using Acridine Orange Fluorescence

Haydn E Edwards
The Research Division of the School of Natural Science, The North East Wales Institute, Kelsterton College, Clwyd, Wales, U. K.
,
Suppiah Navaratnam
The Research Division of the School of Natural Science, The North East Wales Institute, Kelsterton College, Clwyd, Wales, U. K.
,
John C Allen
The Research Division of the School of Natural Science, The North East Wales Institute, Kelsterton College, Clwyd, Wales, U. K.
,
Glyn O Phillips
The Research Division of the School of Natural Science, The North East Wales Institute, Kelsterton College, Clwyd, Wales, U. K.
› Author Affiliations
Further Information

Publication History

Received 13 November 1979

Accepted 31 January 1980

Publication Date:
13 July 2018 (online)

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Summary

A new method to estimate the heparin neutralising capacity of protamine has been investigated. The technique has been applied to two commercial heparin preparations tested against the W.H.O. 1st International Reference preparation of protamine.

The technique is based on the fluorescence of acridine orange dye and the selective binding of heparin with protamine. A titration procedure has been devised and the two variations of the assay are compared and evaluated. The titration is rapid, accurate and convenient.

Keywords

Heparin - Protamine - Acridine orange - Fluorescence - Titration
 

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