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Thromb Haemost 1972; 27(02): 196-204
DOI: 10.1055/s-0038-1649356
Originalarbeiten — Original Articles — Travaux Originaux
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Reaction Characteristics of Antiprothrombin Antibody on the Activation of Prothrombin (Sheep)[*]

Q. Z Hussain   **
1   Department of Biochemistry, Maulana Azad Medical College and associated hospitals, New Dehli, India
,
S Chaudhuri   ***
1   Department of Biochemistry, Maulana Azad Medical College and associated hospitals, New Dehli, India
› Author Affiliations
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Publication History

Publication Date:
29 June 2018 (online)

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Summary

1. Prothrombin was purified by a series of steps and subsequently on DEAE-Cellu- lose chromatography and specific antibody to pure prothrombin was prepared.

2. The effect of Anti-prothrombin antibody was tested on the activation of prothrombin. The generated thrombin was assayed for its clotting function as well as for esterase activity on TAMe (Tosyl-Arginine-Methyl ester) as substrate.

3. Anti-prothrombin antibody inhibits the clotting function of generated thrombin completely but its esterase function is only partially affected.

* Preliminary work was presented at the XIII Congress of the International Society of Hematology, Munich, 1970.


** Present address : Department of Immunochemistry, Indian Statistical Institute, 203, B. T. Road, Calcutta - 35, India.


*** Present address: Department of Medicine, University of Nebraska College of Medicine, Omaha, Nebraska U. S. A.


 

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