Studies on Blood Coagulation Factor V

Summary

It is confirmed that a protein from Russell’s viper venom has a direct activating action on human as well as bovine factor V.

Unactivated human factor V has a molecular weight as estimated by gel filtration of ∼410,000, after activation the molecular weight is ∼110,000, thus suggesting the dissociation of a tetramer.

The approximative molecular weight figures for unactivated and activated bovine factor V are 400,000 and 195,000, resp. This suggests the dissociation into a dimer accompanying the process of activation.

This article forms part of the Ph. D. thesis of the first author that was presented in March 1970 at the University of Leiden.

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