Isolation and Preliminary Characterization of a Vitamin K Dependent Peptide from Human Prothrombin^[*]

Summary

A peptide containing the vitamin K dependent Ca²⁺ binding region of human prothrombin has been isolated by adsorption of the tryptic digest of the native protein on barium citrate. Its aminoacid composition has been determined. The N- and C-terminal residues have been characterized. The obtained results are showing some slight differences with the corresponding bovine peptide. Since they were not strictly identical for two different preparations, the eventuality of a molecular heterogeneity is discussed.

^* Presented at the Vth Congress of the International Society on Thrombosis and Haemostasis, Paris, July, 1975.

^* ** Groupe U 15 de l’Institut National de la Santé et de la Recherche Médicale, Laboratoire Associé au Centre National de la Recherche Scientifique.

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