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Thromb Haemost 1974; 32(02/03): 685-694
DOI: 10.1055/s-0038-1647738
Original Article
Schattauer GmbH

Frog Thrombocytes Aggregation and the Release Reaction

Ronald A. Stiller   *
1   Department of Biology, Boston University, Boston, Massachusetts 02215
,
Frank A. Belamarich
1   Department of Biology, Boston University, Boston, Massachusetts 02215
,
David Shepro
1   Department of Biology, Boston University, Boston, Massachusetts 02215
› Author Affiliations
Further Information

Publication History

Received 29 July 1974

Accepted 19 September 1974

Publication Date:
30 June 2018 (online)

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Summary

Frog thrombocytes were shown to aggregate in response to thrombin, collagen, and adenosine triphosphate (ATP). Microscopic observations indicated that collagen and thrombin induced aggregates were cell-to-cell in nature and not due solely to passive adherence to collagen or fibrin strands.

The ability of ATP to promote aggregation suggested that the release of this nucleotide might be the mechanism by which frog thrombocytes aggregate ; however, there was no evidence to support this hypothesis. Enzymatic hydrolysis of ATP by hexokinase had no effect on thrombin-induced aggregation, and ATP could not be identified in the supernatants of aggregated thrombocytes. Nevertheless, the ability of these cells to aggregate in response to these aggregating agents indicates that significant parallels may exist between platelet aggregation and the aggregation of non-mammalian thrombocytes.

* Present address: Department of Microbiology, Harvard School of Public Health, 667 Huntington Avenue, Boston, Massachusetts 02115.


 

  • References

  • 1 Belamarich F. A, Fusari M. H, Shepro D, Kien M. 1966; In vitro studies of aggregation of non-mammalian thrombocytes. Nature 212: 1579.
    CrossrefPubMedGoogle Scholar
  • 2 Belamarich F. A, Shepro D, Kien M. 1968; ADP is not involved in thrombin- induced aggregation of thrombocytes of non-mammalian vertebrates. Nature 220: 509.
    CrossrefPubMedGoogle Scholar
  • 3 Belamarich F. A, Simoneit L. W. 1973; Aggregation of duck thrombocytes by 5-hydroxytryptamine. Micro vascular Research 06: 229.
    PubMedGoogle Scholar
  • 4 Belamarich F. A, Stiller R. A, Shepro D. 1973; The release reaction: Is it a common mechanism in all types of hemostatic cells ?. Series Haematologica 06: 418.
    PubMedGoogle Scholar
  • 5 Born G VR. 1965; Platelets in thrombogenesis : Mechanism and inhibition of platelet aggregation. Annals of the Royal College of Surgeons. England 36: 200.
    PubMedGoogle Scholar
  • 6 Born G VR. 1970; Observations on the change in shape of blood platelets brought about by adenosine diphosphate. The Journal of Physiology 209: 487.
    CrossrefPubMedGoogle Scholar
  • 7 Born G VR, Foulks J, Michel F, Sharp D. E. 1972; Reversal of the rapid morphological reaction of platelets. The Journal of Physiology 225: 27P.
    PubMedGoogle Scholar
  • 8 Day H. J, Holmsen H, Hovig T. 1969; Subcellular particles of human platelets. A biochemical and electron-microscopic study with particular referance to the influence of fractionation techniques Scandinavian Journal of Haematology Supplement 07: 3.
    PubMedGoogle Scholar
  • 9 Diamant B, Krüger P. G. 1967; Histamine release from isolated rat peritoneal mast cells induced by adenosine—5’—triphosphate. Acta Physiologica Scandinavia 71: 291.
    CrossrefPubMedGoogle Scholar
  • 10 Diamant B, Peterson C. 1971; The metabolism of monosaccharides in isolated rat mast cells and its influence on histamine release induced by adenosine-—5’—triphosphate. Acta Physiologica Scandinavia 83: 324.
    CrossrefPubMedGoogle Scholar
  • 11 Feinberg H, Belamarich F, Born G VR. 1973; Transphosphorylation in human and rabbit platelet suspensions. Federation Proceedings 32: 220 Abs..
    PubMedGoogle Scholar
  • 12 Guccione M. A, Packham M. A, Kinlough-Rathbone R. L, Mustard J. F. 1971; Reactions of 14C-ADP and 14C-ATP with washed platelets from rabbits. Blood 37: 542.
    PubMedGoogle Scholar
  • 13 Haslam R. J. 1964; Role of adenosine diphosphate in the aggregation of human blood- platelets by thrombin and by fatty acids. Nature 202: 765.
    CrossrefPubMedGoogle Scholar
  • 14 Haslam R. J. 1967. Mechanisms of blood platelet aggregation. In: Johnson S. A, Seegers W. H. (eds.) Physioloqy of Hemostasis and Thrombosis. Charles C. Thomas; Springfield: 88.
    Google Scholar
  • 15 Hayem G. 1879; Recherches sur l’évolution des hématies dans le sang de l’homme et des vertébrés. Archive de Physiologie normale et pathologique 2e Serie. 06: 201.
    PubMedGoogle Scholar
  • 16 Holmsen H, Day H. J, Storm E. 1969; Adenine nucleotide metabolism of blood platelets VI. Subcellular localization of nucleotide pools with different functions in the platelet release reaction. Biochimica Biophysica Acta 186: 254.
    PubMedGoogle Scholar
  • 17 Holmsen H, Storm E, Day H. J. 1972; Determination of ATP and ADP in blood platelets: A modification of the firefly luciferase assay for plasma. Analytical Biochemistry 46: 489.
    CrossrefPubMedGoogle Scholar
  • 18 Holmsen H, Weiss H. 1972; Further evidence for a deficient storage poole of adenine nucleotides in platelets from some patients with thrombocytopathia—”storage pool disease”. Blood 39: 197.
    PubMedGoogle Scholar
  • 19 Kien M, Belamarich F. A, Shepro D. 1971; Effect of adenosine and related compounds on thrombocyte and platelet aggregation. American Journal of Physiology 220: 604.
    PubMedGoogle Scholar
  • 20 Pringle H, Tait J. 1910; Natural arrest of hemorrhage in the tadpole. Journal of Physiology 40: vi.
    PubMedGoogle Scholar
  • 21 Sugiyama K. 1971; Significance of ATP-splitting activity of rat peritoneal mast cells in the histamine release induced by exogenous ATP. Japanese Journal of Pharmacology 21: 531.
    CrossrefPubMedGoogle Scholar