Download PDF
Thromb Haemost 1978; 39(03): 555-563
DOI: 10.1055/s-0038-1646728
Original Article
Schattauer GmbH Stuttgart

A Rapid Chromatographic Method for Quantitation of High Molecular Weight Fibrinogen Derivatives in Plasma

Vincenzo Musumeci
The Laboratory of Blood Coagulation, Department of Internal Medicine, the Catholic University, Rome, Italy
,
Dolly Culasso
The Laboratory of Blood Coagulation, Department of Internal Medicine, the Catholic University, Rome, Italy
,
Paolo Boni
The Laboratory of Blood Coagulation, Department of Internal Medicine, the Catholic University, Rome, Italy
› Author Affiliations
Further Information

Publication History

Received 18 October 1977

Accepted 14 December 1977

Publication Date:
12 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

High molecular weight fibrinogen derivatives were precipitated from 3 ml of plasma by 0.88 M ammonium sulphate. The resuspended precipitate was analyzed by chromatography on a 2.5 × 30 cm glass bead Bio-Gel 5 M column by using a flow rate of 60 cm/hr and continuous effluent monitoring at O. D. 280 nm. Complete elution of fibrinogen occurred in 50 min. Elution profile was handled according to the chromatographic plate theory analysis. Results obtained by our method were not significantly different from those obtained by conventional chromatography.

 

  • References

  • 1 Alkjaersig N, Roy L, Fletcher A. 1973; Analysis of gel exclusion chromatographic data by chromatographic plate theory analysis: application to plasma fibrinogen chromatography. Thrombosis Research 3: 525
    CrossrefPubMedGoogle Scholar
  • 2 Andrews P. 1965; The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochemical Journal 96: 595
    CrossrefPubMedGoogle Scholar
  • 3 Donati MB, Verhaeghe R, Culasso D, Vermylen J. 1976; Molecular size distribution of fibrinogen derivatives formed in vitro and in vivo: chromatographic study. Thrombosis and Haemostasis (Stuttg.) 36: 14
    Article in Thieme ConnectPubMedGoogle Scholar
  • 4 Fletcher A, Alkjaersig N, Roy L, Owens O. 1973; Early detection of blood hypercoagulable states and early thromboembolic lesions in man. Proceedings of the Third Technicon International Symposia 4: 8-1-8-8
    PubMedGoogle Scholar
  • 5 Fletcher AP. 1970; Fibrinogen proteolysis in vivo. Thrombosis et Diathesis Haemorrhagica (Stuttg.) 39: 249
    PubMedGoogle Scholar
  • 6 Godal HC, Abildgaard U. 1966; Gelation of soluble fibrin in plasma by ethanol. Scandinavian Journal of Haematology 3: 342
    PubMedGoogle Scholar
  • 7 Graeff H, Wiedemann A, Hugo R, Von Hafter R. 1976; Amount and distribution pattern of soluble fibrin monomer complexes during the early puerperium. American Journal of Obstetrics and Gynecology 124: 21
    CrossrefPubMedGoogle Scholar
  • 8 Latallo ZS. 1975; Formation and detection of fibrinogen derived complexes. Thrombosis et Diathesis Haemorrhagica (Stuttg.) 34: 611
    PubMedGoogle Scholar
  • 9 Niewiarowski S, Gurewich V. 1971; Laboratory identification of intravascular coagulation: the SDPS test for the detection of fibrin monomer and fibrin degradation products. Journal of Laboratory and Clinical Medicine 77: 665
    PubMedGoogle Scholar
  • 10 Sachs D, Painter E. 1972; Improved flow rates with porous sephadex gels. Science 175: 781
    CrossrefPubMedGoogle Scholar
  • 11 Weber K, Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulphate-poly acrilamide gel electrophoresis. Journal of Biological Chemistry 244: 4406
    PubMedGoogle Scholar
  • 12 Winzor DJ. 1969. Analytical gel filtration. In: Leach ASS. (Ed.) Physical principles and techniques of protein chemistry. N. Y. Academic Press; 451
    Google Scholar