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Thromb Haemost 1989; 61(03): 423-428
DOI: 10.1055/s-0038-1646608
Original Article
Schattauer GmbH Stuttgart

Platelet Aggregation in Whole Blood - Studies with a Platelet Counting Technique - Methodological Aspects and Some Applications

C Falcon
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
,
J Arnout
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
,
J Vermylen
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
› Author Affiliations
Further Information

Publication History

Received 07 September 1988

Accepted after revision 10 March 1989

Publication Date:
24 July 2018 (online)

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Summary

We describe a method for measuring platelet aggregation in whole blood by single platelet counting. The importance of a low stirring speed (100 rpm) to obtain agonist-specific aggregation is stressed. Despite this low stirring speed, the sensitivity to agonists equals that of the turbidometric technique in platelet-rich plasma. The optimal concentration of formaldehyde for fixing the aggregates, the effects of storage times and anticoagulant are studied. Applicability to the study of platelet function inhibitors or of inherited platelet function disorders is illustrated. It is concluded that this technique, used under the appropriate conditions, combines the advantage of measuring platelet aggregation in a more physiologtc environment with the advantages of the turbidometric technique such as high sensitivity.

Keywords

Platelet aggregation, whole blood - Platelet aggregation, platelet counting
 

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