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Tierarztl Prax Ausg G Grosstiere Nutztiere 2011; 39(04): 201-204
DOI: 10.1055/s-0038-1623060
Originalartikel
Schattauer GmbH

Nachweis des porzinen Circovirus Typ 2 und des Torque-Teno-Sus-Virus 1 und 2 in Samenproben von Ebern einer österreichischen Besamungsstation[*]

Detection of Porcine Circovirus type 2 and Torque-Teno-Sus-virus 1 and 2 in semen of boars from an Austrian artificial insemination centre
Ch. Lang
1   Klinik für Schweine, Veterinärmedizinische Universität Wien, Wien, Österreich
,
A. Griessler
2   Traunkreis VetClinic, Ried im Traunkreis, Österreich
,
E. Pirker
2   Traunkreis VetClinic, Ried im Traunkreis, Österreich
,
H. Söllner
1   Klinik für Schweine, Veterinärmedizinische Universität Wien, Wien, Österreich
,
J. Segalés
3   Centre de Recerca en Sanitat Animal, UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spanien
4   Departament de Sanitat i d’Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spanien
,
T. Kekarainen
3   Centre de Recerca en Sanitat Animal, UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spanien
,
M. Ritzmann
1   Klinik für Schweine, Veterinärmedizinische Universität Wien, Wien, Österreich
› Author Affiliations
Further Information

Publication History

Eingegangen:24 January 2011

Akzeptiert nach Revision:08 June 2011

Publication Date:
05 January 2018 (online)

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Zusammenfassung

Gegenstand und Ziel: Das porzine Circovirus Typ 2 (PCV-2) und das Torque-teno-Sus-Virus (TTSuV) sind in schweineproduzierenden Ländern häufig nachzuweisen. Beide Erreger können sowohl horizontal als auch vertikal übertragen werden und Ebersamen könnte ein wichtiges Übertragungsmedium darstellen. Ziel der Studie war die Abklärung der Prävalenz dieser beiden Viren in Samenproben von Ebern. Material und Methoden: Von 100 Ebern einer Besamungsstation wurde jeweils eine Samenprobe mittels quantitativer Real-Time-PCR auf PCV-2 und mittels konventioneller PCR auf TTSuV-1 und TTSuV-2 untersucht. Ergebnisse: Nur bei einem Eber der Rasse Piétrain war ein positives PCV-2-Resultat festzustellen. TTSuV-1 ließ sich in vier Samenproben, TTSuV-2 in fünf Proben nachweisen. Ein Eber wies eine Koinfektion mit beiden TTSuV-Genotypen auf. Alle TTSuV-positiven Proben stammten von Piétrain-Ebern. Schlussfolgerung und klinische Relevanz: In der vorliegenden Studie wurde erstmals in Österreich TTSuV im Samen nachgewiesen. Die Prävalenz sowohl von TTSuV als auch von PCV-2 war gering. Die klinische Relevanz einer gleichzeitigen Kontamination des Samens mit beiden Viren ist nicht klar.

Summary

Objective: Porcine circovirus type 2 (PCV-2) and torque-teno-sus-viruses (TTSuV) are ubiquitous in all pig-producing countries. Both pathogens are transmitted horizontally as well as vertically, and semen could be an important means of transmission. Aim of this study was the investigation of the prevalence of these two viruses in boar semen. Material and methods: Single semen samples from 100 boars from an artificial insemination centre were analysed by quantitative real time PCR for PCV2 and by conventional PCR for TTSuV-1 and TTSuV-2. Results: Only one Piétrain boar of age of 47 months showed a measurable PCV-2 quantity of 7.56 × 103/ml semen. Four samples were PCR-positive for TTSuV-1, and five for TTVSu-2, while in a single co-infection both TTSuVs were detected.All TTSuV-positive samples originated from Piétrain boars. Conclu sion and clinical relevance: This study represents the first descrip tion of TTSuV presence in semen in Austria. The prevalence of TTSuV as well as PCV-2 was low. The clinical relevance of a simultaneous contamination of boar semen with both pathogens remains unclear.

Schlüsselwörter

PCV-2 - TTSuV-1 - TTSuV-2 - Schwein

Key words

PCV-2 - TTSuV-1 - TTSuV-2 - pig

* Herrn Prof. Dr. Karl Heinritzi zum 65. Geburtstag gewidmet.


 

  • Literatur

  • 1 Ciacci-Zanella JR, Zanella EL, Locatelli ML, Brambatti JL, Simon NL, Coldebella M. Detection of porcine circovirus 2 in semen collected from naturally infected boars studs in Brazil. Proceedings of 5th International Symposium on Emerging and Re-emerging Pig Diseases. 2007: 94.
    PubMedGoogle Scholar
  • 2 Guerin B, Pozzi N. Viruses in boar semen: detection and clinical as well as epidemiological consequences regarding disease transmission by artificial insemination. Theriogenology 2005; 63: 556-572.
    CrossrefPubMedGoogle Scholar
  • 3 Kauffold J, Melzer F, Henning K, Schulze K, Leiding C, Sachse K. Prevalence of chlamydiae in boars and semen used for artificial insemination. Theriogenology 2006; 65: 1750-1758.
    CrossrefPubMedGoogle Scholar
  • 4 Kekarainen T, Lopez-Soria S, Segales J. Detection of swine Torque teno virus genogroups 1 and 2 in boar sera and semen. Theriogenology 2007; 68: 966-971.
    CrossrefPubMedGoogle Scholar
  • 5 Kekarainen T, Sibila M, Segales J. Prevalence of swine Torque teno virus in post-weaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs in Spain. J Gen Virol 2006; 87: 833-837.
    CrossrefPubMedGoogle Scholar
  • 6 Kim J, Han DU, Choi C, Chae C. Differentiation of porcine circovirus (PCV)-1 and PCV-2 in boar semen using a multiplex nested polymerase chain reaction. J Virol Methods 2001; 98: 25-31.
    CrossrefPubMedGoogle Scholar
  • 7 Kim J, Han DU, Choi C, Chae C. Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested polymerase chain reaction. J Vet Med Sci 2003; 65: 741-744.
    CrossrefPubMedGoogle Scholar
  • 8 Lang C, Söllner H, Barz A, Ladinig A, Langhoff R, Weissenboeck H, Kekarainen T, Segales J, Ritzmann M. Untersuchung zur Verbreitung des Torque Teno-Virus beim Schwein in Österreich. Berl Münchn Tierärztl Wschr 2011; 124: 10-15.
    PubMedGoogle Scholar
  • 9 Larochelle R, Bielanski A, Muller P, Magar R. PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen. J Clin Microbiol 2000; 38: 4629-4632.
    PubMedGoogle Scholar
  • 10 Leary TP, Erker JC, Chalmers ML, Desai SM, Mushahwar IK. Improved detection systems for TT virus reveal high prevalence in humans, non-human primates and farm animals. J Gen Virol 1999; 80: 2115-2120.
    CrossrefPubMedGoogle Scholar
  • 11 Madec F, Rose N, Grasland B, Cariolet R, Jestin A. Post-weaning multisystemic wasting syndrome and other PCV2-related problems in pigs: a 12-year experience. Transbound Emerg Dis 2008; 55: 273-283.
    CrossrefPubMedGoogle Scholar
  • 12 Madson DM, Patterson AR, Ramamoorthy S, Pal N, Meng XJ, Opriessnig T. Reproductive failure experimentally induced in sows via artificial insemination with semen spiked with porcine circovirus type 2. Vet Pathol 2009; 46: 707-716.
    CrossrefPubMedGoogle Scholar
  • 13 Madson DM, Ramamoorthy S, Kuster C, Pal N, Meng XJ, Halbur PG, Opriessnig T. Infectivity of porcine circovirus type 2 DNA in semen from experimentally-infected boars. Vet Res 2009; 40: 10.
    CrossrefPubMedGoogle Scholar
  • 14 Maes D, Nauwynck H, Rijsselaere T, Mateusen B, Vyt P, de Kruif A, Van Soom A. Diseases in swine transmitted by artificial insemination: an overview. Theriogenology 2008; 70: 1337-1345.
    CrossrefPubMedGoogle Scholar
  • 15 Martinez L, Kekarainen T, Sibila M, Ruiz-Fons F, Vidal D, Gortazar C, Segales J. Torque teno virus (TTV) is highly prevalent in the European wild boar (Sus scrofa). Vet Microbiol 2006; 118: 223-229.
    CrossrefPubMedGoogle Scholar
  • 16 Mateusen B, Maes DG, Van Soom A, Lefebvre D, Nauwynck HJ. Effect of a porcine circovirus type 2 infection on embryos during early pregnancy. Theriogenology 2007; 68: 896-901.
    CrossrefPubMedGoogle Scholar
  • 17 McIntosh KA, Harding JC, Parker S, Ellis JA, Appleyard GD. Nested polymerase chain reaction detection and duration of porcine circovirus type 2 in semen with sperm morphological analysis from naturally infected boars. J Vet Diagn Invest 2006; 18: 380-384.
    CrossrefPubMedGoogle Scholar
  • 18 Niel C, Diniz-Mendes L, Devalle S. Rolling-circle amplification of Torque teno virus (TTV) complete genomes from human and swine sera and identification of a novel swine TTV genogroup. J Gen Virol 2005; 86: 1343-1347.
    CrossrefPubMedGoogle Scholar
  • 19 Pogranichniy RM, Jepsen RJ, Sorden S, Evans LE, Stammer R, Yoon KJ. Effect of intrauterine challenge of PCV2 on sows and fetuses. Proceedings of 4th International Symposium on Emerging and Re-emerging Pig Diseases. 2003: 197.
    PubMedGoogle Scholar
  • 20 Rose N, Blanchard P, Cariolet R, Grasland B, Amenna N, Oger A, Durand B, Balasch M, Jestin A, Madec F. Vaccination of porcine circovirus type 2 (PCV2)-infected sows against porcine Parvovirus (PPV) and Erysipelas: effect on post-weaning multisystemic wasting syndrome (PMWS) and on PCV2 genome load in the offspring. J Comp Pathol 2007; 136: 133-144.
    CrossrefPubMedGoogle Scholar
  • 21 Schmoll F, Lang C, Steinrigl AS, Schulze K, Kauffold J. Prevalence of PCV2 in Austrian and German boars and semen used for artificial insemination. Theriogenology 2008; 69: 814-821.
    CrossrefPubMedGoogle Scholar
  • 22 Segales J, Allan GM, Domingo M. Porcine circovirus diseases. Anim Health Res Rev 2005; 6: 119-142.
    CrossrefPubMedGoogle Scholar
  • 23 Segales J, Larsen L, Wallgren P, Rose N, Grau-Roma L, Sibila M, Fraile L, Casal J, Baekbo P. What do we know on epidemiology, control and prevention of porcine circovirus diseases?. Proceedings of 5th International Symposium on Emerging and Re-emerging Pig Diseases. 2007; 35-38.
    PubMedGoogle Scholar
  • 24 Wallgren P, Blomqvist G, Thorén P, Elander J, Wallgren M. Incidence of PCV2 in semen collected at Swedish boar stations. Proceedings of 20th IPVS Congress. Durban, South Africa: 2008: 62.
    Google Scholar