Bestimmung von Serum-Amyloid A, Haptoglobin und Fibrinogen als Entzündungsparameter nach Kastration von Hengsten^[*]

Zusammenfassung

Gegenstand und Ziel: Gegenstand der vorliegenden Studie war die Frage, ob sich die Akute-Phase-Proteine Serum-Amyloid A, Haptoglobin und Fibrinogen nicht nur für die Entzündungsdiagnostik eignen, sondern auch eine Aussage über das Ausmaß einer Entzündung und den postoperativen Heilungsverlauf zulassen. Material und Methoden: Es wurden zwei Gruppen von Hengsten verglichen, die alle einer bedeckten Kastration in Allgemeinanästhesie unterzogen wurden. Pferde in Gruppe 1 (n = 13, mittleres Alter drei Jahre) zeigten postoperativ keine bis geringgradige Wundheilungsstörungen. Dagegen traten bei Pferden der Gruppe 2 (n = 12, durchschnittliches Alter zwei Jahre) mittel- bis hochgradige Wundheilungsstörungen auf (geschwollener, vermehrt warmer Wundbereich, Exsudation, teilweise eitrig, Schmerzhaftigkeit, Lahmheit). Die Probanden wurden unmittelbar vor und sechs, 12, 24, 48 und 72 Stunden sowie sieben Tage nach der Operation untersucht. Ergebnisse: Die Körperinnentemperatur differierte ebenso wie die Gesamtleukozytenzahl zwischen den Gruppen deutlich. Die Konzentration von Serum-Amyloid A stieg bei allen Pferden innerhalb von 24 Stunden nach der Kastration signifikant an. Alle Probanden wiesen 48 Stunden nach dem Eingriff einen Anstieg der Fibrinogenkonzentration im Plasma auf. Die Haptoglobinmessung ergab eine Erhöhung im Plasma innerhalb von 48 Stunden in Gruppe 1 und innerhalb von 72 Stunden in Gruppe 2. Schlussfolgerung: Sowohl die Verlaufskontrolle der klinischen Parameter als auch der ausgewählten Akute- Phase-Proteine zeigte als Reaktion auf die Operation und eine nachfolgende Entzündung deutlich ansteigende Werte. Klinische Relevanz: Während bei der Kastration die Bestimmung der klinischen Parameter ausreichend erscheint, bietet sich eine kombinierte Messung von Serum-Amyloid A und Haptoglobin oder Fibrinogen zur Einstufung von unbekannten Entzündungsherden und -symptomen an.

Summary

Objective: The aim of this study was to investigate whether the acute phase proteins (APP) serum amyloid A, haptoglobin and fibrinogen could be useful in diagnosis of the degree of inflammation in horse. Material and methods: We compared two groups of horses which underwent castration under general anaesthesia. Horses in group 1 showed no to mild disorder of wound healing, while horses in the second group displayed moderately to severely impaired wound healing. Group 1 included 13 stallions with a mean age of three years. In these horses cardinal symptoms of inflammation were absent or only mild during the observation period. Group 2 consisted of 12 stallions with a mean age of two years. All of these horses showed all cardinal symptoms of inflammation such as wound swelling, heat, exsudation (sometimes putrid), pain and even lameness. The horses were examined directly before and six, 12, 24, 48, 72 and 168 hours post surgery. Results: Body temperature as well as WBC differed significantly between the two groups. Serum amyloid A rose in serum samples of all horses within 24 hours post surgery. Plasma concentration of fibrinogen rose within 48 hours post surgery in all horses. Within 48 hours in group 1 and 72 hours in group 2 there was also an increase in haptoglobin concentration. Conclusions: In all examined parameters distinct changes in response to surgery and inflammation could be found. For body temperature, WBC, haptoglobin and fibrinogen there was a marked difference between group 1 and 2. Clinical relevance: In case of castration a continous registration of clinical parameters may be sufficient. A combination of serum amyloid A and haptoglobin or fibrinogen measurements provides a valuable tool to clarify patients exhibiting unclear symptoms of inflammation.

Schlüsselwörter:

Akute-Phase-Proteine - Pferd - Kastration - Serum-Amyloid A (SAA) - Fibrinogen - Haptoglobin - Labordiagnostik

Keywords

Acute phase protein - equine castration - serum amyloid A - fibrinogen - haptoglobin - laboratory diagnostic

^* Herrn Prof. Dr. med. vet. habil. Olof Dietz zum 80. Geburtstag gewidmet.

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