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Thromb Haemost 1999; 81(06): 869-873
DOI: 10.1055/s-0037-1614590
Letters to the Editor
Schattauer GmbH

Free and Total Platelet Glycoprotein IIb/IIIa Measurement in Whole Blood by Quantitative Flow Cytometry during and after Infusion of c7E3 Fab in Patients Undergoing PTCA

Nathalie Hézard
1   From the Laboratoire Central d’Hématologie and, Reims, France
,
Damien Metz
2   Service de Cardiologie, CHU Robert Debré, Reims, France
,
Pierre Nazeyrollas
2   Service de Cardiologie, CHU Robert Debré, Reims, France
,
Philippe Nguyen
1   From the Laboratoire Central d’Hématologie and, Reims, France
,
Gérard Simon
1   From the Laboratoire Central d’Hématologie and, Reims, France
,
Sylvie Daliphard
1   From the Laboratoire Central d’Hématologie and, Reims, France
,
Chantal Droullé
1   From the Laboratoire Central d’Hématologie and, Reims, France
,
Jacques Elaerts
2   Service de Cardiologie, CHU Robert Debré, Reims, France
,
Gérard Potron
1   From the Laboratoire Central d’Hématologie and, Reims, France
› Author Affiliations
Further Information

Publication History

Received 03 June 1998

Accepted after resubmission 17 February 1999

Publication Date:
09 December 2017 (online)

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Summary

A quantitative flow cytometry assay was used to evaluate the ex vivo kinetics of c7E3 Fab platelet effect in 16 patients undergoing PTCA treated with abciximab and compared with aggregometry assay. Immunolabeling of platelets was directly assessed on whole blood, using in parallel two monoclonal antibodies (Mabs) raised against GPIIIa, Mab1, the binding of which is inhibited by c7E3 Fab, and Mab2, the binding of which is not affected by c7E3 Fab. We found a severe and sustained inhibition of both GPIIb/IIIa receptors and platelet functions. The inter-individual variation in response to abciximab was low. A significant transient increase at H24 and H48 in the binding of Mab2 was found as an unexpected result, and confirmed in vitro. Results demonstrate that flow cytometry is a reliable method in agreement with aggregation. In addition, our results show that it is a standardized tool and a time-saving technique.

 

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