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Thromb Haemost 2000; 84(03): 453-459
DOI: 10.1055/s-0037-1614044
Commentary
Schattauer GmbH

Fibrin Down-regulates LPS- and PMA-induced Tissue Factor Expression by Blood Mononuclear Cells

Maria Rosaria Rossiello
1   From the Dipartimento di Scienze Biomediche e Oncologia Umana, Sezione di Patologia Generale, Bari, Italy
,
Alessandra Italia
1   From the Dipartimento di Scienze Biomediche e Oncologia Umana, Sezione di Patologia Generale, Bari, Italy
,
Anna M. Stramaglia
1   From the Dipartimento di Scienze Biomediche e Oncologia Umana, Sezione di Patologia Generale, Bari, Italy
,
Loreto Gesualdo
2   Dipartimento dell’Emergenza e dei Trapianti d’Organi, Sezione di Nefrologia, University of Bari, Bari, Italy
,
Giuseppe Grandaliano
2   Dipartimento dell’Emergenza e dei Trapianti d’Organi, Sezione di Nefrologia, University of Bari, Bari, Italy
,
Francesco P. Schena
2   Dipartimento dell’Emergenza e dei Trapianti d’Organi, Sezione di Nefrologia, University of Bari, Bari, Italy
,
Nicola Semeraro
1   From the Dipartimento di Scienze Biomediche e Oncologia Umana, Sezione di Patologia Generale, Bari, Italy
,
Mario Colucci
1   From the Dipartimento di Scienze Biomediche e Oncologia Umana, Sezione di Patologia Generale, Bari, Italy
› Author AffiliationsThis work was supported in part by a grant from Italian MURST (Ministero della Università e della Ricerca Scientifica e Tecnologica).
Further Information

Publication History

Received 28 December 1999

Accepted after resubmissiopn 10 April 2000

Publication Date:
14 December 2017 (online)

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Summary

Several studies indicate that fibrin may play a functional role in inflammation by modulating a variety of cellular functions. We investigated the effect of fibrin on tissue factor (TF) production by blood mononuclear cells (MNC). Citrated human blood was recalcified and incubated at 37° C for 1-4 h. The resulting clot was lysed by the addition of tissue plasminogen activator (t-PA) and MNC were isolated by density gradient centrifugation. A control blood sample was processed in the same way but omitting calcium addition and clot formation. Clot-and blood-derived MNC did not express detectable TF activity and antigen whatever the incubation time. Clot-derived MNC, however, generated on average 5 fold less TF (activity and antigen) than control cells, when stimulated with lipopolysaccharide (LPS, 1 µg/ml) for 3 h at 37° C. A reduced TF response of clot-derived cells was also observed at mRNA level as indicated by RT-PCR and in situ hybridization. The effect was dependent on the incubation time within the clot, could not be reversed by enhancing LPS concentration or by adding serum, and was maintained if LPS was replaced by the tumor promoter PMA. A reduced TF response was also found when washed MNC were incorporated for 1 h at 37° C within purified fibrin but not when the cells were incubated with fibrinogen, thrombin or fibrin split products alone, indicating that contact with fibrin was responsible for the inhibition of TF production. Fibrin-induced down-regulation of TF response to LPS and PMA by MNC may represent a negative feed-back aimed at limiting excessive blood clotting activation in immuneinflammatory diseases.

Key words

Fibrin - leukocytes - coagulation - tissue factor
 

  • References

  • 1 Dejana ES, Colella LR, Languino LR, Balconi G, Corbascio GC, Marchisio PC. Fibrinogen induces adhesion, spreading and microfilament organization of human endothelial cells in vitro. J Cell Biol 1987; 104: 1403-11.
    CrossrefPubMedGoogle Scholar
  • 2 Ribes JA, Francis WC, Wagner DD. Fibrin induces release of von Willebrand factor from endothelial cells in vitro. J Clin Invest 1987; 79: 117-23.
    CrossrefPubMedGoogle Scholar
  • 3 Kaplan KL, Mather T, De Marco L, Solomon S. Effect of fibrin on endothelial cell production of prostacyclin and tissue plasminogen activator. Arteriosclerosis 1989; 09: 43-9.
    CrossrefPubMedGoogle Scholar
  • 4 Qi J, Kreutzer DL, Piela-Smith TH. Fibrin induction of ICAM-1 expression in human vascular endothelial cells. J Immunol 1997; 158: 1880-6.
    PubMedGoogle Scholar
  • 5 Colucci M, Balconi G, Lorenzet R, Pietra A, Locati D, Donati MB, Semeraro N. Cultured human endothelial cells generate tissue factor in response to endotoxin. J Clin Invest 1983; 71: 1893-6.
    CrossrefPubMedGoogle Scholar
  • 6 Levin EG, Loskutoff DJ. Cultured bovine endothelial cells produce both urokinase and tissue-type plasminogen activators. J Cell Biol 1982; 94: 631-6.
    CrossrefPubMedGoogle Scholar
  • 7 Van Mourik JA, Lawrence DA, Loskutoff DJ. Purification of an inhibitor of plasminogen activator (antiactivator) synthesized by endothelial cells. J Biol Chem 1984; 259: 14914-21.
    PubMedGoogle Scholar
  • 8 Colucci M, Paramo JA, Collen D. Generation in plasma of a fast-acting inhibitor of plasminogen activator in response to endotoxin stimulation. J Clin Invest 1985; 75: 818-24.
    CrossrefPubMedGoogle Scholar
  • 9 Contrino J, Goralnick S, Qi J, Hair G, Rickles FR, Kreutzer DL. Fibrin induction of tissue factor expression in human vascular endothelial cells. Circulation 1997; 96: 605-13.
    PubMedGoogle Scholar
  • 10 Niemetz J, Muhlfelder T, Chierego ME, Troy B. Procoagulant activity of leukocytes. Ann NY Acad Sci 1977; 283: 208-17.
    CrossrefPubMedGoogle Scholar
  • 11 Edwards RL, Rickles FR. Macrophage procoagulants. In: Progress in haemostasis and thrombosis. Spaet TH. ed. New York: Grune & Stratton; 1984: 183-201.
    Google Scholar
  • 12 Semeraro N, Colucci M. Tissue Factor in health and disease. Thromb Haemost 1997; 78: 759-64.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 13 Perez RL, Roman J. Fibrin enhances the expression of IL-1b by human peripheral blood mononuclear cells. J Immunol 1995; 154: 1879-87.
    PubMedGoogle Scholar
  • 14 Scarpati EM, Wen D, Broze Jr. GJ, Miletich JP, Flandermeyer RR, Siegel NR, Sadler JE. Human tissue factor: cDNA sequence and chromosome localization of the gene. Biochemistry 1987; 26: 5234-8.
    CrossrefPubMedGoogle Scholar
  • 15 Nakajima-Iijima S, Hamada H, Reddy P, Kakunaga T. Molecular structure of the human cytoplasmic β-actin gene: interspecies homology of sequence in the introns. Proc Natl Acad Sci USA 1985; 82: 6133-7.
    CrossrefPubMedGoogle Scholar
  • 16 Boyum A. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol 1976; 05 (Suppl) 9-15.
    PubMedGoogle Scholar
  • 17 Semeraro N, Biondi A, Lorenzet R, Locati D, Mantovani A, Donati MB. Direct induction of tissue factor synthesis by endotoxin in human macrophages from diverse anatomical sites. Immunology 1983; 50: 529-35.
    PubMedGoogle Scholar
  • 18 Yam LT, Li CY, Crosby WH. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol 1971; 55: 283-90.
    CrossrefPubMedGoogle Scholar
  • 19 Di Minno G, Cerbone AM, Mattioli PL, Turco S, Iovine C, Mancini M. Functionally thrombasthenic state in normal platelets following the administration of ticlopidine. J Clin Invest 1985; 75: 328-38.
    CrossrefPubMedGoogle Scholar
  • 20 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156-9.
    PubMedGoogle Scholar
  • 21 Gesualdo L, Di Paolo S, Milani S, Pinzani M, Grappone C, Ranieri E, Pannarale G, Schena FP. Expression of platelet derived growth factor receptors in normal and diseased human kidney: an immunohistochemical and in situ hybridization study. J Clin Invest 1994; 94: 50-8.
    CrossrefPubMedGoogle Scholar
  • 22 Niemetz J, Marcus A. The stimulatory effect of platelets and platelet membranes on the procoagulant activity of leukocytes. J Clin Invest 1974; 54: 1437-43.
    CrossrefPubMedGoogle Scholar
  • 23 Steinemann S, Ulevitch RJ, Mackman N. Role of the lipopolysaccharide (LPS)-binding protein/CD14 pathway in LPS induction of tissue factor expression in monocytic cells. Arterioscler Thromb 1994; 14: 1202-9.
    CrossrefPubMedGoogle Scholar
  • 24 Tersinien C, Ramani M, Ollivier V, Khechai F, Vu T, Hakim J, de Prost D. Endotoxin-induced tissue factor in human monocytes is dependent upon protein kinase C activation. Thromb Haemost 1993; 70: 800-6.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 25 Van der Logt CPE, Dirven RJ, Reitsma PH, Bertina RM. Expression of tissue factor and tissue factor pathway inhibitor in monocytes in response to bacterial lipopolysaccharide and phorbolester. Blood Coag Fibrinol 1994; 05: 211-20.
    CrossrefPubMedGoogle Scholar
  • 26 Tersinien C, Ollivier V, Khechai F, Ramani M, Hakim J, de Prost D. Protein Tyrosine Kinase Activation is required for LPS and PMA induction of tissue factor mRNA in human blood monocytes. Thromb Haemost 1995; 73: 413-20.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 27 Ritchie H, Jamieson A, Booth NA. Thrombin modulates synthesis of plasminogen activator inhibitor type-2 by human peripheral blood monocytes. Blood 1995; 86: 3428-35.
    PubMedGoogle Scholar
  • 28 Semeraro N, Montemurro P, Chetta G, Altomare DF, Giordano D, Colucci M. Increased procoagulant activity of peripheral blood monocytes in human and experimental jaundice. Gastroenterology 1989; 96: 892-8.
    CrossrefPubMedGoogle Scholar
  • 29 Osterud B. Tissue factor: a complex biological role. Thromb Haemost 1997; 78: 755-8.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 30 Terry CM, Callahan KS. Protein kinase C regulates cytokine-induced tissue factor transcription and procoagulant activity in human endothelial cells. J Lab Clin Med 1996; 127: 81-93.
    CrossrefPubMedGoogle Scholar
  • 31 Altieri DC. Xa receptor EPR-1. Faseb J 1995; 09: 860-5.
    CrossrefPubMedGoogle Scholar
  • 32 Hou L, Howells GL, Kapas S, Macey MG. The protease-activated receptors and their cellular expression and function in blood-related cells. Br J Haematol 1998; 10: 1-9.
    PubMedGoogle Scholar
  • 33 Nelson JC, Lerner RG. Detection of FXIIIa (active fibrin stabilizing factor) in normal plasma. Blood 1978; 52: 581-91.
    PubMedGoogle Scholar
  • 34 Altieri DC, Bader R, Mannucci PM, Edgingtom TS. Oligospecificity of the cellular adhesion receptor Mac-1 encompasses an inducible recognition specificity for fibrinogen. J Cell Biol 1988; 107: 1893-900.
    CrossrefPubMedGoogle Scholar