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Thromb Haemost 2000; 83(05): 759-768
DOI: 10.1055/s-0037-1613905
Review Article
Schattauer GmbH

Thromboxane Synthase Has the Same Pattern of Expression as Platelet Specific Glycoproteins during Human Megakaryocyte Differentiation

Natacha Vitrat
1   From INSERM U 362, Laboratoire associé n°5 du comité de Paris de la Ligue Nationale contre le Cancer, Institut Gustave Roussy, Villejuif, France
,
Rémi Letestu
1   From INSERM U 362, Laboratoire associé n°5 du comité de Paris de la Ligue Nationale contre le Cancer, Institut Gustave Roussy, Villejuif, France
,
Aline Massé
1   From INSERM U 362, Laboratoire associé n°5 du comité de Paris de la Ligue Nationale contre le Cancer, Institut Gustave Roussy, Villejuif, France
,
Vladimir Lazar
1   From INSERM U 362, Laboratoire associé n°5 du comité de Paris de la Ligue Nationale contre le Cancer, Institut Gustave Roussy, Villejuif, France
,
William Vainchenker
1   From INSERM U 362, Laboratoire associé n°5 du comité de Paris de la Ligue Nationale contre le Cancer, Institut Gustave Roussy, Villejuif, France
,
Najet Debili
1   From INSERM U 362, Laboratoire associé n°5 du comité de Paris de la Ligue Nationale contre le Cancer, Institut Gustave Roussy, Villejuif, France
› Author AffiliationsWe thank Dr. J. L. Nichol (Amgen, Thousand Oaks, CA) for the gift of PEG-rhuMGDF, and stem cell factor; R. McEver (Oklahoma City, OK) for providing Tab monoclonal antibody. This work was supported by grants from the Institut National de la Santé et de la Recherche Médicale, Comité de Paris de la Ligue contre le Cancer and the Institut Gustave Roussy. N. V. is supported by an Amgen grant. We thank Pr B. Forget for improving the english manuscript.
Further Information

Publication History

Received 25 September 1998

Accepted after resubmission 27 August 1999

Publication Date:
08 December 2017 (online)

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Summary

Regulation of the platelet formation process is poorly understood. It has been shown that p45NF-E2 deficient mice have a profound defect in platelet formation and recently the first platelet/megakaryocytic gene regulated by NF-E2, thromboxane synthase (TXS), has been identified. In this study, we investigated TXS expression as a model of a gene regulated by NF-E2 during MK differentiation. Megakaryocytic cells derived from blood CD34+ cells were purified according to their stage of maturation on the basis of expression of CD34, CD41a and CD42a, permitting to define different stages in MK differentiation. By means of real-time quantitative RT-PCR, we could determine that the level of TXS increased during differentiation in parallel with the expression of c-mpl and GPIIb (CD41). However, amounts of TXS transcripts increased about 1.6-fold more than that of GPIIb or c-mpl transcripts during maturation. Expression of TXS and MK specific proteins such as CD41a, CD42a and vWF was also correlated in maturing MKs. In addition, staining by anti-TXS antibody of proplatelet bearing MKs was not increased in comparison to that observed in mature MK, suggesting that TXS is not upregulated during platelet formation. In addition, we investigated whether TXS and cyclooxygenase could be involved in platelet formation by adding aspirin into the cultures. No significant decrease of platelet production was observed.

In conclusion, this study shows that TXS is coordinately expressed with the other platelet proteins during MK differentiation but is not directly involved in platelet formation.

Keywords

Megakaryocyte - TXS
 

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