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Thromb Haemost 2000; 83(02): 316-321
DOI: 10.1055/s-0037-1613805
Rapid Communication
Schattauer GmbH

Monitoring of Aspirin (ASA) Pharmacodynamics with the Platelet Function Analyzer PFA-100®

Monika Homoncik
1   From the Department of Clinical Pharmacology-TARGET, Vienna University Hospital School of Medicine, Vienna, Austria
,
Bernd Jilma
1   From the Department of Clinical Pharmacology-TARGET, Vienna University Hospital School of Medicine, Vienna, Austria
,
Nicole Hergovich
1   From the Department of Clinical Pharmacology-TARGET, Vienna University Hospital School of Medicine, Vienna, Austria
,
Petra Stohlawetz
2   Univ. Clinic for Blood Group Serology and Transfusion Medicine, Vienna University Hospital School of Medicine, Vienna, Austria
,
Simon Panzer
2   Univ. Clinic for Blood Group Serology and Transfusion Medicine, Vienna University Hospital School of Medicine, Vienna, Austria
,
Wolfgang Speiser
3   Clinical Institute of Medical and Chemical Laboratory Diagnostics, Vienna University Hospital School of Medicine, Vienna, Austria
› Author Affiliations
Further Information

Publication History

Received 20 July 1999

Accepted 19 October 1999

Publication Date:
11 December 2017 (online)

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Summary

Background

Anti-platelet drug therapy is currently performed without monitoring, because the established method of platelet aggregometry is cumbersome. The recently developed platelet function analyzer PFA-100® measures shear stress dependent, collagen epinephrine (CEPI) and collagen adenosine diphosphate (CADP) induced platelet plug formation. As the PFA-100 provides a valuable tool to detect patients with platelet dysfunction more efficiently and cost-effectively than aggregometry, we investigated its potential to monitor the efficacy of aspirin treatment.

Methods

All healthy volunteers (n = 10) received a fractionated infusion of L-aspirin to establish individual dose-response curves. Further, in a randomized, double-blind, placebo controlled two-way cross over study the same volunteers received either 50 or 100 mg aspirin/day p.o. for a period of 11 days to determine the day-to-day variability CEPI induced closure time (CT) under constant intake of low dose aspirin, and to compare the efficacy of those two doses.

Results

Intra- and intersubject variability of CEPI-CT averaged 9% and 22%, respectively. Seven volunteers exceeded the maximum of CEPI-CT (>300 s) already after infusion of 100 mg L-aspirin. Intake of 100 mg of aspirin elicited a more rapid onset of effect than 50 mg, which was only significant on days 3 and 4 of aspirin intake. The aspirin induced CEPI-CT prolongation correlated positively with basal CEPI-CT values (r = 0.86; p = 0.001) and were strongly dependent on von Willebrand Factor levels (r = -0.9; p = 0.001).

Conclusion

Thus, the PFA-100 system appears suitable to demonstrate an aspirin-induced platelet effect in a longitudinal study, and may be adequate to monitor a patient’s compliance. However, prospective trials have to be conducted to demonstrate whether the EPI-CT achieved under ASA-intake has predictive value for cardiovascular outcome.

Keywords

Aspirin - platelet function analyzer - PFA-100 - alpha2-integrin
 

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